PMID: 16113891Aug 23, 2005Paper

Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden

Memórias do Instituto Oswaldo Cruz
Edward José de OliveiraDirce Mary Correia Lima

Abstract

IgM-ELISA is an immunoenzymatic method useful for detection of IgM antibodies against a fraction of Schistosoma mansoni adult worm antigen (AWA) that is soluble in trichloroacetic acid (AWA-TCA). This method was applied to three groups of individuals with different clinical and epidemiological characteristics, and the results compared with those obtained by other diagnostic methods: immunofluorescence test for detection of IgM antibodies (IgM-IFT) or IgG antibodies (IgG-IFT), ELISA for detection of IgG antibodies (IgG-ELISA), and two parasitological methods, Kato-Katz and miracidium hatching. The IgM-ELISA presented a sensitivity of 98%, when the parasitologic fecal examination was defined as reference diagnostic method, and a specificity of 98 and 97.3%, respectively for the group of clinically healthy individuals and other helminth carriers. A comparative analysis between the results of IgM-ELISA and those obtained by other serologic tests showed a good degree of agreement, with Kappa indices ranging from 0.95 to 0.98. The diagnostic efficacy of 97.8%, as determined with schistosomiasis patients with low parasitic burden, suggests the excellent performance of the IgM-ELISA and its usefulness for the diagnosis of schistosomias...Continue Reading

References

Jan 1, 1993·Transactions of the Royal Society of Tropical Medicine and Hygiene·M J DoenhoffJ Bain
Apr 1, 1996·The American Journal of Tropical Medicine and Hygiene·D EngelsB Gryseels
May 4, 1999·Memórias do Instituto Oswaldo Cruz·B Alarcón de NoyaO Noya
Jul 16, 2002·Memórias do Instituto Oswaldo Cruz·Herminia Yohko KanamuraLuiz Candido de Souza Dias
Apr 18, 2003·Cadernos de saúde pública·Edward José de OliveiraRicardo Mário de Carvalho Ciaravolho
Aug 1, 1992·Parasitology Today·S J de Vlas, B Gryseels

❮ Previous
Next ❯

Citations

Jan 24, 2009·International Ophthalmology·Daniel Vítor Vasconcelos-SantosCarlos Eduardo Pavesio
Mar 4, 2008·Transactions of the Royal Society of Tropical Medicine and Hygiene·Edward José de OliveiraMário Hiroyuki Hirata
Jul 18, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·M C C Espirito-SantoR C B Gryschek
Apr 16, 2013·Acta Tropica·Lydia LeonardoRaman Velayudhan
Feb 20, 2007·Memórias do Instituto Oswaldo Cruz·Edward José de OliveiraMário Hiroyuki Hirata
Aug 20, 2010·Memórias do Instituto Oswaldo Cruz·André Ricardo Ribas FreitasLuiz Jacintho Silva
Mar 19, 2014·The American Journal of Tropical Medicine and Hygiene·Maria Cristina Carvalho do Espírito-SantoRonaldo Cesar Borges Gryschek
Aug 1, 2015·Clinical Microbiology Reviews·Kosala G A D WeerakoonDonald P McManus
Feb 19, 2008·Cadernos de saúde pública·Cybele GargioniHermínia Yohko Kanamura
Apr 4, 2019·Memórias do Instituto Oswaldo Cruz·Vanessa Silva-MoraesPaulo Marcos Zech Coelho
Feb 9, 2018·PLoS Neglected Tropical Diseases·Derick N M OsakunorFrancisca Mutapi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.