Efficacy of chitosan, a natural polysaccharide, against Cryptosporidium parvum in vitro and in vivo in neonatal mice

Experimental Parasitology
Mohamed MammeriKarim Tarik Adjou

Abstract

Cryptosporidiosis is a zoonotic disease caused by species in the genus Cryptosporidium. In young ruminants, Cryptosporidium parvum causes economically significant disease with mild to severe clinical signs and occasional death. The typical clinical course in animals aged 1-3 weeks old is acute diarrhoea. Currently there are no available treatments that are fully effective against cryptosporidiosis in either humans or animals. Therefore there is a critical need for the development of new therapeutic agents. We adapted two in vitro culture systems (HCT-8 and Caco-2 cell lines) for C. parvum infection to investigate the "anticryptosporidial" activity of two chitosans; Chitosan NAG and Chitosan Mix. Chitosan-a naturally-occurring polysaccharide compound-has been found to be active against a variety of diseases, possessing both antimicrobial and anticancer properties. We investigated both chitosan's toxicity and effects on C. parvum in the two in vitro models. To evaluate chitosan's effects on oocyst shedding in vivo, CD-1 neonate mice were orally inoculated with C. parvum oocysts (Iowa strain), treated with chitosan, and compared to infected non-treated animals. Paromomycin, a classical drug used in veterinary medicine, was used as...Continue Reading

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Citations

Jun 13, 2019·Parasitology Research·Shahira A AhmedPanagiotis Karanis
Aug 10, 2020·Zoonoses and Public Health·Carolina Ortiz PinedaRegina Maura Bueno Franco
Nov 13, 2020·Frontiers in Microbiology·Mohamed MammeriKarim Tarik Adjou
Dec 20, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saravut Satitsri, Chatchai Muanprasat
Mar 2, 2021·International Journal for Parasitology. Drugs and Drug Resistance·Astrid Dominguez-UscangaJuan E Andrade Laborde
Apr 4, 2021·Nanomaterials·Hamdan I AlMohammedHossein Mahmoudvand
Dec 5, 2019·Veterinary Parasitology : Regional Studies and Reports·Mohamed MammeriKarim Tarik Adjou
Apr 15, 2020·International Journal of Biological Macromolecules·Priscilla Barbosa Sales AlbuquerqueLuana Cassandra Breitenbach Barroso Coelho
Sep 4, 2021·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Howayda Said Fouad MoawadAmira Abd El-Lateef Saleh Ali

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