Abstract
The combination of fusidic acid and rifampicin has a demonstrated synergistic effect against methicillin-resistant Staphylococcus aureus (MRSA), including planktonic and biofilm-related organisms. However, the in vitro efficacy of other combinations of oral anti-MRSA antibiotics in biofilm models has not been established. The antibacterial activity of fusidic acid, linezolid, rifampicin, and minocycline against 33 biofilm-embedded MRSA isolates in low susceptibility and high resistance breakpoint concentrations was investigated using the 3-[4, 5-dimethyl-2-thiazolyl]-2, 5-diphenyl-2H-tetrazolium-bromide staining method. The compounds were further examined to determine their antibacterial efficacies in combination. The optical density ratio (ODr) was used to evaluate the antibacterial effects of these antibiotics, and the results indicate higher survival rates of MRSA on biofilm. A biofilm-positive phenotype (determined using the crystal violet stain) was defined as an optical density ≥ 0.17 at 492 nm, and strong biofilm formation was defined as an optical density ≥ 1.0. One-third of the MRSA isolates demonstrated weak biofilm formation, and two-thirds demonstrated strong biofilm formation. At low concentrations, linezolid alone...Continue Reading
References
Jul 7, 1999·Vaccine·S K KairoM J Corbel
Dec 8, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·R M Donlan
Apr 5, 2002·Clinical Microbiology Reviews·Rodney M Donlan, J William Costerton
Oct 4, 2002·The New England Journal of Medicine·Alice S Prince
Dec 4, 2002·American Journal of Infection Control·Naomi P O'gradyUNKNOWN Healthcare Infection Control Practices Advisory Committee
Jan 9, 2003·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Naomi P O'GradyUNKNOWN Healthcare Infection Control Practices Advisory Committee
Oct 25, 2003·Antimicrobial Agents and Chemotherapy·Issam RaadWilliam Costerton
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·Samuel A ShelburneRichard J Hamill
Oct 16, 2004·The New England Journal of Medicine·Werner ZimmerliPeter E Ochsner
Mar 24, 2005·International Journal of Antimicrobial Agents·D SchillaciV Sciortino
Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Robin K PettitMarilyn L Horton
Oct 19, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Dennis L StevensUNKNOWN Infectious Diseases Society of America
Dec 27, 2005·Antimicrobial Agents and Chemotherapy·Raphael SaginurKaram Ramotar
Jul 15, 2006·The Journal of Antimicrobial Chemotherapy·Anjum S KakaDaniel M Musher
Aug 22, 2006·American Journal of Surgery·Charles E EdmistonJonathan B Towne
Oct 24, 2006·The Journal of Antimicrobial Chemotherapy·Houssem HmoudaLetaief Jemni
Dec 21, 2006·Folia Microbiologica·E WalenckaB Rózalska
Mar 3, 2007·Journal of Clinical Microbiology·Eoghan O'NeillJames P O'Gara
Mar 3, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C A AboltinsP A Stanley
Mar 14, 2007·Antimicrobial Agents and Chemotherapy·Issam RaadRay Hachem
Apr 30, 2008·Archives of Internal Medicine·Joshua PerlrothLoren G Miller
Dec 20, 2008·International Journal of Antimicrobial Agents·Jakub KwiecińskiKinga Wójcik
Citations
Apr 11, 2013·Antimicrobial Agents and Chemotherapy·Julia BauerFrançoise Van Bambeke
Jan 11, 2014·Drug Development and Industrial Pharmacy·Niloofar Moghadas-SharifBizhan Malaekeh-Nikouei
Feb 4, 2016·Biofouling·Haiying CuiLin Lin
Feb 18, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Wen-Sen LeeYung-Wei Lin
May 27, 2014·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Wen-Sen LeeShio-Shin Jean
Dec 24, 2013·The Journal of Arthroplasty·Patrick O'TooleXian Long Zhang
Jul 22, 2014·Letters in Applied Microbiology·P-H ElchingerP Michaud
Jan 28, 2014·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Patrick O'TooleXian Long Zhang
May 6, 2014·Frontiers in Microbiology·Audrey CharleboisMarie Archambault
Apr 8, 2016·Expert Opinion on Pharmacotherapy·Concepcion Perez-JorgeJaime Esteban
May 20, 2016·Infectious Diseases·Guillaume G AubinStéphane Corvec
May 27, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Alaa AbouelfetouhMoustafa El-Nakeeb
May 29, 2015·PloS One·Talita Signoreti GrazianoKarina Cogo-Müller
Nov 5, 2016·Journal of the Mechanical Behavior of Biomedical Materials·Fahad Alam, Kantesh Balani
May 2, 2018·Antimicrobial Agents and Chemotherapy·Wafi SialaFrançoise Van Bambeke
Aug 10, 2016·Antimicrobial Agents and Chemotherapy·Douglas Fraser-PittDeborah O'Neil
Aug 21, 2013·Antimicrobial Agents and Chemotherapy·Hung-Jen TangYin-Ching Chuang
May 22, 2019·Current Molecular Medicine·Ritika RanaAnoop Kumar
Feb 15, 2014·Journal of Applied Microbiology·P D M de MatosM O Pereira
Sep 12, 2017·International Journal of Clinical Practice·Nicholas D BarwellUNKNOWN Scottish Diabetes Foot Action Group
Dec 19, 2019·Frontiers in Pharmacology·Andreia Borges ScriboniKarina Cogo-Müller
Oct 23, 2020·Drug Discovery Today·Kushal VanamalaArun K Iyer
Feb 2, 2021·Curēus·Mohammad Zubair