Efficacy of combinations of colistin with other antimicrobials involves membrane fluidity and efflux machinery

Infection and Drug Resistance
E ArmengolM Vinas

Abstract

Despite its use was abandoned several decades ago, the polycationic peptide colistin has become the last hope to treat severe infections caused by multidrug-resistant Gram-negative bacteria. Thus, the development of colistin resistance may seriously compromise the efficacy of treatment. Moreover, colistin has high toxicity being dose dependent. A potentially effective strategy to avoid resistance may be to combine colistin with other antimicrobials. This may help in the rescue of old antimicrobials and in reducing toxic undesired effects. Antimicrobial susceptibility determination, efflux machinery function measurements in different conditions and measurement of inhibition of the extrusion by colistin were performed. Moreover, modifications of anisotropy of the membranes by using fluorescent dyes was accomplished. Sub-inhibitory concentrations of colistin have a synergistic effect with several antimicrobials that act intracellularly (targeting protein synthesis and DNA replication). This effect was demonstrated through the uptake increases of acridine orange. in Pseudomonas aeruginosa, Escherichia coli and Acinetobacter baumanii but also in an intrinsically colistin-resistant species as Serratia marcescens. Measurements of the ...Continue Reading

Citations

Feb 26, 2021·Expert Opinion on Drug Discovery·Josep M Sierra, Miguel Viñas
Mar 7, 2021·Antibiotics·Francesco BuonocoreSimona Picchietti
Oct 15, 2021·Infectious Diseases and Therapy·Jacinda C Abdul-MutakabbirZahra Kassamali Escobar

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BETA
light microscopy

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