Efficacy of different DNA and MVA prime-boost vaccination regimens against a Rift Valley fever virus (RVFV) challenge in sheep 12 weeks following vaccination

Veterinary Research
Gema LorenzoAlejandro Brun

Abstract

The aim of this work was to evaluate the immunogenicity and efficacy of DNA and MVA vaccines encoding the RVFV glycoproteins Gn and Gc in an ovine model of RVFV infection. Adult sheep of both sexes were challenged 12 weeks after the last immunization and clinical, virological, biochemical and immunological consequences, were analyzed. Strategies based on immunization with homologous DNA or heterologous DNA/MVA prime-boost were able to induce a rapid in vitro neutralizing antibody response as well as IFNγ production after in vitro virus specific re-stimulation. In these animals we observed reduced viremia levels and less clinical signs when compared with mock-immunized controls. In contrast, sheep inoculated with a homologous MVA prime-boost showed increased viremia correlating with the absence of detectable neutralizing antibody responses, despite of inducing cellular responses after the last immunization. However, faster induction of neutralizing antibodies and IFNγ production after challenge were found in this group when compared to the mock vaccinated group, indicative of a primed immune response. In conclusion, these results suggest that vaccination strategies based on DNA priming were able to mount and maintain specific an...Continue Reading

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Citations

May 16, 2019·Antimicrobial Agents and Chemotherapy·Belén BorregoAlejandro Brun
Aug 9, 2020·Transboundary and Emerging Diseases·Alexis C R HostePatricia Sastre
Sep 4, 2019·Expert Opinion on Biological Therapy·Tetsuro Ikegami
Aug 15, 2020·NPJ Vaccines·Eva Calvo-PinillaJavier Ortego
Jun 13, 2020·Frontiers in Veterinary Science·Andrea Louise KroekerWilliam C Wilson
Feb 13, 2021·NPJ Vaccines·Eva Calvo-PinillaJavier Ortego
Apr 3, 2020·Molecular Pharmaceutics·Juan Gonzalez-ValdiviesoF Javier Arias

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Datasets Mentioned

BETA
DQ380208.1

Methods Mentioned

BETA
CISA
ELISA

Software Mentioned

GraphPad

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