PMID: 2119210Mar 1, 1990Paper

Efficacy of direct plating media for recovering Listeria monocytogenes from foods

International Journal of Food Microbiology
D A GoldenL R Beuchat

Abstract

Studies were done to evaluate 14 direct plating media for their suitability to recover Listeria monocytogenes strain Scott A from pasteurized whole milk, chocolate ice cream mix, Brie cheese and raw cabbage. Healthy cells were inoculated into foods to achieve viable populations of 10(2), 10(4), or 10(6) cells/ml(g). Bind's Acriflavine Agar, Trypaflavine Nalidixic Acid Serum Agar, Listeria Transport Enrichment Agar, Doyle and Schoeni Selective Enrichment Agar (DSSEA) and Modified DSSEA were not suitable for recovering L. monocytogenes from milk and Brie cheese and were therefore not evaluated as direct plating media for recovering the organism from ice cream mix and cabbage. McBride Listeria Agar (MLA), Gum Base Nalidixic Acid Tryptone Soya Agar (GBNTSA), Modified Despierres Agar (MDA) and Modified MLA (MMLA) performed best for recovering all inoculum populations from milk and ice cream mix. Enumeration of L. monocytogenes on several test media was complicated by the growth of large numbers of background microflora present in cabbage and Brie cheese, especially at the lowest test inoculum (10(2)/g). Generally, complete recovery of L. monocytogenes from Brie cheese and cabbage was attained on media when the inoculum population wa...Continue Reading

References

Aug 1, 1977·The Journal of Applied Bacteriology·P Mavrothalassitis
Oct 1, 1986·Canadian Journal of Microbiology·L R BeuchatD E Conner
Feb 14, 1985·The New England Journal of Medicine·D W FlemingA L Reingold
Jul 26, 1985·JAMA : the Journal of the American Medical Association
Feb 1, 1981·The Journal of Applied Bacteriology·J Watkins, K P Sleath
Jan 27, 1983·The New England Journal of Medicine·W F SchlechC V Broome

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Citations

Jun 1, 1989·International Journal of Food Microbiology·R E Brackett, L R Beuchat
Jun 17, 2000·International Journal of Food Microbiology·R Firstenberg-Eden, L A Shelef
Apr 27, 2000·The New England Journal of Medicine·P AureliS Salmaso
Nov 29, 2005·Journal of Microbiological Methods·Robin L T ChurchillJ Christopher Hall
Mar 22, 2003·FEMS Immunology and Medical Microbiology·Rijkelt R Beumer, Wilma C Hazeleger

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