Efficacy of DNA vaccines expressing the type F botulinum toxin Hc fragment using different promoters

Vaccine
Amit P JathoulHelen S Garmory

Abstract

DNA vaccines which expressed the Hc fragment of the Clostridium botulinum type F neurotoxin (BoNT/F Hc) fused to a signal peptide downstream of four different eukaryotic promoters were prepared. Subsequently, the immunogenicity of the DNA vaccines and protection afforded in mice against challenge with 10(4) MLD of type F botulinum toxin was evaluated. The DNA vaccine containing the human ubiquitin gene (UbC) promoter induced the highest BoNT/F Hc-specific antibody concentration following two intramuscular immunisations and afforded 90% protection against challenge. The results from this study indicate that the selection of promoter used in DNA vaccination studies may be of importance in designing optimised vaccines.

Citations

May 15, 2010·Journal of Biomedicine & Biotechnology·Penelope KorakaAlbert D M E Osterhaus
Sep 2, 2010·Biologicals : Journal of the International Association of Biological Standardization·Yun-Zhou YuZhi-Wei Sun
Oct 20, 2009·Vaccine·Leonard A Smith
Oct 6, 2009·Vaccine·Shishir Kumar GuptaBashir Akhlaq Akhoon
Jul 16, 2008·Toxicon : Official Journal of the International Society on Toxinology·Gabriela Azofeifa-CorderoAlberto Alape-Girón
Jun 10, 2006·Infectious Disease Clinics of North America·Rodrigo G VillarKaren M Davenport
May 17, 2005·Biochemical and Biophysical Research Communications·Hyang-Min ByunYu-Kyoung Oh
May 28, 2011·International Immunopharmacology·Rajeev Nagill, Sukhbir Kaur
Sep 5, 2017·Toxins·Grace Sundeen, Joseph T Barbieri
Oct 11, 2008·Clinical and Vaccine Immunology : CVI·Yun-Zhou YuZhi-Wei Sun

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.