PMID: 9546797Apr 18, 1998Paper

Efficacy of fusion peptide homologs in blocking cell lysis and HIV-induced fusion

AIDS Research and Human Retroviruses
K A SilburnC C Curtain

Abstract

Contrary to earlier reports, we have found that tri- and hexapeptides analogous or homologous with segments of the 23-residue N-terminal fusion sequence (FS) of the viral transmembrane glycoprotein gp41 (residues 517-539) did not significantly inhibit HIV-1-induced syncytium formation, using an uninfected cell-infected cell fusion assay. In contrast, we found that the high molecular weight apolipoprotein A-1 and a 23-residue analog of the FS, with the phenylalanine residues at positions 524 and 527 replaced with alanine residues, were effective inhibitors. Although the tripeptides were ineffective as inhibitors of syncytium formation, we found a number of them inhibited red cell lysis induced by the synthetic peptide AVGIGALFLGFLGAAGSTMGARS (based on the HIV-1 gp41 FS). This effect was also seen with apolipoprotein A-1. The Ala524,527 analog of the fusion sequence could not be tested in this system because it was hemolytic. We concluded that the smaller peptides were effective inhibitors of hemolysis because they interfered with pore formation by the fusion sequence peptide, either by disrupting the pores or by preventing the peptide from adopting the alpha-helical conformation found in the pores. On the other hand, membrane fu...Continue Reading

References

Jan 1, 1979·Annual Review of Biochemistry·J A Op den Kamp
Aug 25, 1992·Biochimica Et Biophysica Acta·P W MobleyL M Gordon
Feb 1, 1992·AIDS Research and Human Retroviruses·P Li, C J Burrell
Nov 1, 1990·AIDS Research and Human Retroviruses·R J OwensR W Compans
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E O FreedR Risser
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P L EarlB Moss
Jun 1, 1990·AIDS Research and Human Retroviruses·R KiernanD McPhee
Dec 1, 1988·Journal of Virological Methods·R E KiernanR R Doherty
Jun 1, 1995·AIDS Research and Human Retroviruses·L M GordonP W Mobley
Jun 9, 1995·Biochimica Et Biophysica Acta·P W MobleyL M Gordon
Nov 1, 1993·AIDS Research and Human Retroviruses·L M GordonA J Waring
Dec 1, 1995·Nature Structural Biology·M LuP S Kim

❮ Previous
Next ❯

Citations

Aug 6, 2008·The Journal of Biological Chemistry·Stefania GaldieroMassimiliano Galdiero
Feb 24, 2011·Virology Journal·Lilia I MelnikCindy A Morris
Feb 8, 2013·Journal of Peptide Science : an Official Publication of the European Peptide Society·Stefania GaldieroMassimiliano Galdiero

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.