Efficacy of human islet isolation from the tail section of the pancreas for the possibility of living donor islet transplantation

Transplantation
Shinichi MatsumotoJ A Reems

Abstract

Islet transplantation is on the rise for the treatment of type 1 diabetes. Apparent donor shortages could be alleviated through use of living donor pancreata. A critical issue for using a section of pancreas from living donors is whether islet yields would be sufficient for transplantation. After obtaining human pancreata, islets were isolated from the head section (n=20, head group), tail section (n=23, tail group) or whole pancreas (n=24, whole group). Islets were isolated by enzymatic digestion followed by purification, then assessed for yields, purity, morphology, functionality, and insulin content. Fifteen of twenty cases (75%) in the head group, all cases (100%) in the tail group, and 23 of 24 cases (96%) in the whole group were successfully completed for islet isolation. Islet yield per gram pancreas was significantly higher in the tail group compared with both the head and whole groups (head, 1,472+/-326 IE/g; tail, 4,256+/-574 IE/g; whole, 2,424+/-506 IE/g). Total islet yield from the head group was significantly lower compared with both tail and whole groups (head, 75,016+/-18,933 IE; tail, 197,469+/-28,236 IE; whole, 208,207+/-43,414 IE), and the tail group showed similar islet yield to the whole group. The whole gro...Continue Reading

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Citations

Jul 5, 2005·Journal of Hepato-biliary-pancreatic Surgery·Shinichi Matsumoto, Koichi Tanaka
Jun 7, 2008·Journal of Hepato-biliary-pancreatic Surgery·Hirofumi Noguchi, Shinichi Matsumoto
Jan 2, 2007·Transplantation·Shinichi MatsumotoKoichi Tanaka
Apr 4, 2007·Postgraduate Medical Journal·P SrinivasanN D Heaton
May 24, 2012·Biotechnology & Genetic Engineering Reviews·M'Balu A WebbRoger F James
Dec 24, 2005·Expert Opinion on Biological Therapy·Shinichi MatsumotoCamillo Ricordi
Apr 9, 2009·Biopolymers·S Janette WilliamsCory Berkland
Nov 30, 2006·Annals of the New York Academy of Sciences·Yasuhiro IwanagaKoichi Tanaka
Dec 18, 2009·Clinical Transplantation·Dan RidgwaySteve White
Oct 7, 2010·Journal of Diabetes·Shinichi Matsumoto
Jan 16, 2007·Drug Metabolism and Pharmacokinetics·Eriko SatoKen-ichi Inui
Oct 2, 2007·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Nicholas OnacaMarlon F Levy
Apr 5, 2020·Journal of Diabetes·Shinichi Matsumoto, Masayuki Shimoda
Oct 20, 2007·Proceedings·Shinichi MatsumotoMarlon Levy
Dec 10, 2020·Nature Communications·Jared Carlson-StevermerKrishanu Saha

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