Efficacy of low concentrations of sodium hypochlorite and low-powered Er,Cr:YSGG laser activated irrigation against an Enterococcus faecalis biofilm

International Endodontic Journal
J E ChristoPeter C Cathro

Abstract

To establish the antibacterial efficacy of low concentrations of sodium hypochlorite with and without Er,Cr:YSGG laser activation on Enterococcus faecalis biofilms in extracted teeth. The root canals of 96 decoronated single-rooted extracted human teeth were prepared to a size 40, 0.06 taper 1 mm beyond the apex. They were mounted within a flow cell, which was sterilized before pumping a nutrient media through the root canals. The flow cell was inoculated with E. faecalis (ATCC 700802) and cultivated for 4 weeks. The root-ends were sealed, and the roots were then subjected to one of six treatment groups: group 1: syringe irrigation (SI) with saline (control) using a 27 -gauge Monoject needle 1 mm from the apex for 2 min; group 2: as for group 1 but with 1% NaOCl; group 3: as for group 1 but with 4% NaOCl; group 4: 0.5% NaOCl irrigation for 15 s followed by laser-activated irrigation (LAI) with four 15-s cycles replenishing the irrigant between cycles; group 5: as for group 4 but with 1% NaOCl as the irrigant; group 6: as for group 4 but with 4% NaOCl as the irrigant. Following treatment, teeth were crushed and viable bacteria were quantitated by serial dilution and plating. The colony-forming unit values were compared between g...Continue Reading

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Citations

Mar 17, 2017·Brazilian Dental Journal·Leila Clarisse HillesheimCleonice Silveira Teixeira
Sep 23, 2016·Brazilian Dental Journal·Helena Suleiman de MacedoWalter Raucci-Neto
Aug 31, 2016·Photomedicine and Laser Surgery·Miguel Rodrigues MartinsNorbert Gutknecht
Sep 20, 2018·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Meltem Küçük, Fatma Kermeoğlu
Nov 30, 2018·International Endodontic Journal·R C D SwimbergheM A Meire
Mar 26, 2016·International Endodontic Journal·S De MeyerR J G De Moor
Dec 10, 2020·Restorative Dentistry & Endodontics·Kimia KhoshrooLobat Tayebi
May 2, 2021·International Endodontic Journal·Rosalie C D SwimbergheMaarten A Meire

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