Efficacy of Newcastle disease virus recombinant expressing avian influenza virus H6 hemagglutinin against Newcastle disease and low pathogenic avian influenza in chickens and turkeys

Avian Diseases
Diana SchröerThomas C Mettenleiter

Abstract

A recombinant Newcastle disease virus (NDV) expressing H6 hemagglutinin (HA) of a low pathogenic avian influenza virus (LPAIV) was generated by reverse genetics (NDVH6). The H6 open reading frame was inserted as an additional transcription unit between the fusion and hemagglutinin-neuraminidase (HN) gene of lentogenic NDV clone 30. Expression of the foreign gene was demonstrated by northern blot, western blot, and indirect immunofluorescence analyses. The protective efficacy against Newcastle disease and avian influenza of subtype H6 was evaluated in 3-wk-old chickens and turkeys. A single vaccination protected specific-pathogen-free (SPF) chickens against a subsequent lethal NDV infection and prevented shedding of AIV after homologous H6 LPAIV infection. Furthermore, vaccinated and AIV-infected animals could be differentiated by detection of AIV nucleoprotein-specific antibodies. Three-week-old commercial turkeys, exhibiting NDV-specific maternal antibodies, were partially protected against a lethal NDV challenge infection. The mortality rate of NDVH6-immunized turkeys was reduced to 40% compared to 90% in unvaccinated birds. After H6 LPAIV infection, shedding in NDVH6-immunized turkeys was only marginally reduced compared to ...Continue Reading

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Citations

Mar 8, 2012·Future Microbiology·Dmitriy Zamarin, Peter Palese
Jul 28, 2016·Human Vaccines & Immunotherapeutics·Rory D de Vries, Guus F Rimmelzwaan
Dec 6, 2016·Veterinary Microbiology·David L Suarez, Mary J Pantin-Jackwood
Nov 21, 2012·Journal of Virology·Madhan Mohan ChellappaV N Vakharia
Apr 23, 2020·Viruses·Muhammad Bashir BelloAbdul Rahman Omar
Dec 28, 2016·Veterinary Microbiology·Kiril M DimitrovPatti J Miller

Methods Mentioned

BETA
PCR
in vitro transcription
electrophoresis
fluorescence microscopy
enzyme-linked immunosorbent assay
transfection
ELISA
ELISAs

Related Concepts

Influenza in Birds
Isohemagglutinins
Newcastle Disease
Newcastle disease virus
Orthomyxovirus Type A, Porcine
Meleagridinae
Vaccines, Attenuated
Semisynthetic Vaccines
Viral Vaccines
Gene Expression Regulation, Viral

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