PMID: 11318756Apr 25, 2001Paper

Efficacy of nonwoven fabric bioreactor immobilized with porcine hepatocytes for ex vivo xenogeneic perfusion treatment of liver failure in dogs

Artificial Organs
K NaruseM Makuuchi

Abstract

We have developed a new bioartificial liver bioreactor filled with porcine hepatocytes immobilized on polyester nonwoven fabric (NWF). In this study, we investigated the efficacy of our hybrid bioartificial liver system incorporating the NWF bioreactors and an immunoglobulin adsorbent column for perfusion treatment in a canine liver failure model. Xenogeneic perfusion treatment for operative canine liver failure models were performed for 3 h, and survival time, intracranial pressure, and blood and cerebrospinal fluid data were documented. Treatment was carried out without obstruction by immunological rejection when immunoglobulin adsorbent columns were used with the NWF bioreactors in combination. Dogs treated with this system exhibited a restricted increase of intracranial pressure and significant compensatory effects on blood and cerebrospinal amino acid imbalances as shown by a significant improvement of Fischer's ratio. On the other hand, relatively low capacity for ammonia elimination was shown as compared with homologous direct hemoperfusion.

References

Jul 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S D LidofskyJ R Lake
Sep 1, 1986·Acta paediatrica Scandinavica·M ArisakaS Yanagawa
Jan 1, 1974·American Journal of Surgery·J E FischerF Deindoerfer
May 1, 1994·Artificial Organs·N L SussmanJ H Kelly
Dec 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A J EllisR Williams

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Citations

Mar 16, 2013·Liver International : Official Journal of the International Association for the Study of the Liver·Anwar A PalakkanJames A Ross

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