Efficacy of proline in the treatment of menopause

Experimental Biology and Medicine
Sun-Young NamHyun-Ja Jeong

Abstract

The amino acids in the placenta have multiple functions; however, the therapeutic effects of proline remain poorly for relief postmenopausal symptoms. The aim of present study was to evaluate the effects of proline in the treatment of menopause using in vitro and in vivo models. We assessed the therapeutic effects and regulatory mechanisms of proline by using MCF-7 estrogen-dependent cells, MG63 osteoblast cells, and ovariectomized mice model. An in vivo study was carried out in eight-week-old sham and ovariectomized group. The ovariectomized mouse was further subdivided into two groups administered orally with 17β-estradiol or proline (10 mg/kg/day) for eight weeks. Proline significantly increased cell proliferation and Ki-67 levels in MCF-7 cells and enhanced cell proliferation, alkaline phosphatase activity, extracellular signal-regulated kinase phosphorylation, and glutamyl-prolyl-tRNA synthetase activation in MG63 cells. The estrogen receptor-β and estrogen-response elements luciferase activity were significantly increased by proline in MCF-7 and MG63 cells. In ovariectomized mice, oral administration of proline (10 mg/kg/day) for eight weeks significantly reduced body and vaginal weights. Proline also significantly increa...Continue Reading

References

Dec 1, 1985·Proceedings of the Society for Experimental Biology and Medicine·P U Dubuc
Mar 15, 1995·Annals of Internal Medicine·J Sánchez-GuerreroG A Colditz
Jun 4, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B L RiggsL J Melton
Aug 2, 2001·The Journal of Biological Chemistry·R K VadlamudiR Kumar
Dec 31, 2002·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P TorricelliA Carpi
Sep 30, 2003·Journal of Clinical Densitometry : the Official Journal of the International Society for Clinical Densitometry·Sami KoltaChristian Roux
Mar 17, 2004·Journal of Pharmaceutical and Biomedical Analysis·Piyali Datta, Debasish Bhattacharyya
Apr 22, 2004·Journal of Ethnopharmacology·Junko Mori-OkamotoHiroyuki Yoshimura
Jun 23, 2005·The Journal of the British Menopause Society·Helen Buckler
Dec 1, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Zhao-Ning JiK W K Tsim
Sep 14, 2007·Brain Research Reviews·Paul MicevychKevin Sinchak
Oct 26, 2007·Journal of Cellular Physiology·Angela RaucciAlka Mansukhani
Jan 5, 2008·Environmental Health Perspectives·Guillermo RimoldiWolfgang Wuttke
Mar 4, 2008·Lancet·Heidi D Nelson
Oct 3, 2009·Reviews in Endocrine & Metabolic Disorders·Ronald C Hamdy
May 21, 2013·International Immunopharmacology·Tony L H ChuCaigan Du
May 30, 2013·Journal of Ginseng Research·Myeong Kuk Shim, Young Joo Lee
Jun 14, 2013·Menopause International·Nick PanayUNKNOWN British Menopause Society and Women's Health Concern
Sep 17, 2013·Nutrition·Na-Ra HanHyun-Ja Jeong
Jun 28, 2014·Climacteric : the Journal of the International Menopause Society·R A LoboR J Baber
Aug 22, 2014·Nature Communications·Kirsi AuroMarkus Perola
Dec 23, 2014·Journal of the International Society of Sports Nutrition·Vinicius Fernandes CruzatPhilip Newsholme
Feb 11, 2015·Human Reproduction·L Lindh-ÅstrandA-C Spetz Holm

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Citations

Aug 18, 2018·Nutrition & Metabolism·Qi ZhaoHong-Wen Deng
Aug 31, 2021·Frontiers in Cell and Developmental Biology·Eduardo J PatriarcaGabriella Minchiotti

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