Efficacy of Recombinase Polymerase Amplification to Diagnose Trypanosoma cruzi Infection in Dogs with Cardiac Alterations from an Endemic Area of Mexico

Vector Borne and Zoonotic Diseases
Matilde Jiménez-CoelloBruno L Travi

Abstract

Chagas disease is a lingering Public Health problem in Latin America with ∼5.7 million people infected with Trypanosoma cruzi. Transmission is still taking place in most countries of the Americas, including the United States. Dogs are frequently infected with T. cruzi and its high infection prevalence is associated with increased risk of Chagas disease in humans. The city of Mérida in the Yucatan peninsula is endemic for Chagas disease and canines are frequently infected with T. cruzi. The objective of this study was to evaluate the performance of a qualitative point of care (POC) molecular test (RPA-LF, recombinase polymerase amplification-lateral flow) developed in our laboratory for identifying infected dogs. We used retrospective samples of dogs that came for consultation because of cardiac alterations and proved to be infected with T. cruzi as determined by enzyme-linked immunosorbent assay (ELISA), Western blot, and quantitative PCR (qPCR). The analytical sensitivity indicated that RPA-LF amplified T. cruzi DNA in samples containing almost equal to one to two parasites per reaction. Serial twofold dilutions of T. cruzi epimastigotes showed that the test had 95% (19/20) repeatability at concentrations of two parasites per ...Continue Reading

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Citations

Feb 5, 2019·Expert Review of Anti-infective Therapy·Julio Alonso-PadillaJoaquim Gascón
Mar 29, 2019·Parasites & Vectors·Ricardo G Maggi, Friederike Krämer
May 13, 2020·Journal of Parasitology Research·Maxwell W WaemaHelena A Ngowi
Dec 2, 2020·Vector Borne and Zoonotic Diseases·Thomas R SheliteBruno L Travi

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
PCR

Software Mentioned

RPA
LF

Related Concepts