Efficacy of Syringe Filtration for the Selective Isolation of Campylobacter from Chicken Carcass Rinse

Journal of Food Protection
Jung-Whan ChonKun-Ho Seo

Abstract

We investigated the efficacy of syringe filtration for selective isolation of Campylobacter from chicken carcass rinse by combining syringe filtration with the conventional culture method. Whole chicken carcass rinses were incubated in Bolton enrichment broth, set aside or subjected to syringe filtration, and streaked on Campy-Cefex agar with or without cefoperazone antibiotic supplement. Compared with the conventional method without filtration, 0.65-μm-pore-size syringe filtration resulted in a significantly higher number of Campylobacter-positive samples (23.8 to 37.5% versus 70.0 to 72.5%; P < 0.05), a lower number of plates contaminated with non-Campylobacter (93.8% versus 6.3 to 26.3%), and a lower growth index (1 = growth of a few colonies; 2 = growth of colonies on about half of the plate; and 3 = growth on most of the plate) for competing microbiota (2.9 to 3.0 versus 1.2 to 1.4). When syringe filtration was applied, agar plates containing the antibiotic had significantly less contamination (6.3% versus 26.3%; P < 0.05) and a lower growth index (1.2 versus 1.4) compared with plates without the antibiotic, although the Campylobacter isolation rate was similar (P > 0.05). Syringe filtration combined with conventional enri...Continue Reading

References

Apr 1, 1988·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·F J BoltonG Parker
Mar 8, 2007·Journal of Food Protection·Alfonso Valdivieso-GarciaKris Rahn
Mar 21, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·G Wilson, L B Aitchison
Nov 11, 2008·Journal of Microbiological Methods·Sueptrakool WisessombatSupayang P Voravuthikunchai
Dec 17, 2009·Journal of Food Protection·Leslie SpeegleOmar A Oyarzabal

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