Efficiency of gold nanoparticles coated with the antimicrobial peptide indolicidin against biofilm formation and development of Candida spp. clinical isolates

Infection and Drug Resistance
Elisabetta de AlteriisEmilia Galdiero

Abstract

This article examines the use of a novel nano-system, gold nanoparticles coated with indolicidin (AuNPs-indolicidin), against pathogenic Candida albicans biofilms. Candida species cause frequent infections owing to their ability to form biofilms, primarily on implant devices. We used an integrated approach, evaluating the effect of AuNPs-indolicidin on prevention and eradication of Candida biofilms formed in multi-well polystyrene plates, with relative gene expression assays. Four biofilm-associated genes (FG1, HWP1, ALS1 and ALS3, and CDR1 and CDR2) involved in efflux pump were analyzed using reverse transcription polymerase chain reaction. Treatment with the nano-complex significantly inhibits the capacity of C. albicans to form biofilms and impairs preformed mature biofilms. Treatment with AuNPs-indolicidin results in an increase in the kinetics of Rhodamine 6G efflux and a reduction in the expression of biofilm-related genes. These data provide a chance to develop novel therapies against nosocomially acquired refractory C. albicans biofilms.

Citations

Apr 10, 2020·Future Microbiology·Taynara Mt de LimaDouglas R Monteiro
May 5, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Kristyna SmerkovaVojtech Adam
Jan 1, 2020·Current Protein & Peptide Science·Sk Abdul Mohid, Anirban Bhunia
Apr 17, 2019·Pharmaceutics·Lucia LombardiStefania Galdiero
Jul 13, 2019·Pharmaceutics·Emilia GaldieroRosa Carotenuto
Sep 5, 2019·Pharmaceutics·Ángela Martin-SerranoF Javier de la Mata
Nov 14, 2019·Pharmaceutics·Marcin MakowskiNuno C Santos
Aug 31, 2019·Biomedical Optics Express·L A Dykman, N G Khlebtsov
Apr 3, 2020·Scientific Reports·Emilia GaldieroStefania Galdiero
Oct 13, 2020·Frontiers in Microbiology·Noel Vera-González, Anita Shukla
Dec 2, 2020·Emerging Topics in Life Sciences·Ioanna Mela, Clemens F Kaminski
Apr 14, 2021·International Journal of Peptide Research and Therapeutics·Majid AnwarAbdul Aleem
May 26, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Hong Kit LimChor Yong Tay
Jul 2, 2021·Applied Microbiology and Biotechnology·Payal GuptaKrishna Mohan Poluri

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Methods Mentioned

BETA
PCR
fluorescence spectroscopy

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