Efficiency of mammalian selenocysteine incorporation.

The Journal of Biological Chemistry
Anupama MehtaPaul R Copeland

Abstract

Five components have thus far been identified that are necessary for the incorporation of selenocysteine (Sec) into approximately 25 mammalian proteins. Two of these are cis sequences, a SECIS element in the 3'-untranslated region and a Sec codon (UGA) in the coding region. The three known trans-acting factors are a Sec-specific translation elongation factor (eEFSec), the Sec-tRNA(Sec), and a SECIS-binding protein, SBP2. Here we describe a system in which the efficiency of Sec incorporation was determined quantitatively both in vitro and in transfected cells, and in which the contribution of each of the known factors is examined. The efficiency of Sec incorporation into a luciferase reporter system in vitro is maximally 5-8%, which is 6-10 times higher than that in transfected rat hepatoma cells, McArdle 7777. In contrast, the efficiency of Sec incorporation into selenoprotein P in vitro is approximately 40%, suggesting that as yet unidentified cis-elements may regulate differential selenoprotein expression. In addition, we have found that SBP2 is the only limiting factor in rabbit reticulocyte lysate but not in transfected rat hepatoma cells where SBP2 is found to be mostly if not entirely cytoplasmic despite having a strong p...Continue Reading

References

Jun 21, 1997·BMJ : British Medical Journal·P A JuddA T Diplock
Mar 27, 2001·EMBO Reports·R M TujebajevaM J Berry
Aug 3, 2001·The EMBO Journal·D J KleinT A Steitz
Jan 14, 2003·Annual Review of Nutrition·Donna M Driscoll, Paul R Copeland
Apr 2, 2003·Molecular Cell·Ann Marie ZavackiMarla J Berry
May 31, 2003·Science·Gregory V KryukovVadim N Gladyshev

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Citations

Jan 18, 2006·Cellular and Molecular Life Sciences : CMLS·K Caban, P R Copeland
Feb 17, 2009·Molecular and Cellular Biochemistry·Filomena G OttavianoJoseph Loscalzo
Jun 22, 2005·Biochimica Et Biophysica Acta·Linda JohanssonElias S J Arnér
Apr 12, 2005·Nature Structural & Molecular Biology·Laurent ChavatteDonna M Driscoll
Sep 21, 2012·The Journal of Biological Chemistry·Jonathan N Gonzalez-FloresPaul R Copeland
Dec 16, 2005·The Journal of Biological Chemistry·Diane E HandyJoseph Loscalzo
Sep 15, 2009·Antioxidants & Redox Signaling·Jesse Donovan, Paul R Copeland
Jun 3, 2009·Molecular Biology and Evolution·Sergi CastellanoAndrew G Clark
Jun 21, 2011·Nucleic Acids Research·Michael E BudimanDonna M Driscoll
Sep 12, 2009·BMC Evolutionary Biology·Jesse Donovan, Paul R Copeland
Dec 27, 2007·Circulation Journal : Official Journal of the Japanese Circulation Society·Filomena G OttavianoJoseph Loscalzo
Apr 26, 2007·RNA·Michael T HowardChristine B Anderson
Jan 3, 2012·The Journal of Nutritional Biochemistry·Shuvojit BanerjeeCharles B Foster
Apr 7, 2009·Biochimica Et Biophysica Acta·Sergi Castellano
Mar 31, 2009·Biochimica Et Biophysica Acta·Lutz Schomburg, Ulrich Schweizer
Jul 15, 2015·Antioxidants & Redox Signaling·Anne-Laure Bulteau, Laurent Chavatte
Nov 6, 2012·Biological & Pharmaceutical Bulletin·Yasumitsu Ogra, Yasumi Anan
Oct 30, 2010·Cell·J Robert Hogg, Stephen P Goff
May 22, 2010·Journal of Molecular Biology·Jesse Donovan, Paul R Copeland
Apr 30, 2013·Journal of Molecular Biology·Nirupama GuptaPaul R Copeland
Dec 14, 2016·Nucleic Acids Research·Noelia Fradejas-VillarMichael T Howard
Mar 1, 2005·The Journal of Biological Chemistry·Diane E HandyJoseph Loscalzo
May 8, 2010·Critical Reviews in Biochemistry and Molecular Biology·Alexey V LobanovVadim N Gladyshev
Jul 27, 2014·The Journal of Biological Chemistry·Sumangala P ShettyPaul R Copeland
Sep 7, 2019·Nutrients·Olivia M GuillinLaurent Chavatte
Jul 28, 2019·The Journal of Biological Chemistry·Wenchao ZhaoUlrich Schweizer

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