PMID: 6171303Nov 27, 1981Paper

Efficiency of T4 DNA ligase-catalyzed end joining after S1 endonuclease treatment on duplex DNA containing single-stranded portions

Biochimica Et Biophysica Acta
K Shishido, T Ando

Abstract

Covalently closed-circular, superhelical SV4O DNA was used in all experiments. EcoRI endonuclease- and HpaII endonuclease-generated unit-length linear duplex DNAs were digested with S1 endonuclease under the conditions where single-stranded CNA was completely converted into the acid-soluble form. These were subjected to an end-to-end joining test with T4 DNA ligase. The ligation efficiency was significantly lower than that of the flush-ended linear duplex DNAs which were generated by both HpaI endonuclease digestion and the matching up of EcoRI-generated sticky end with Escherichia coli DNA polymerase I (Klenow fraction). However, the ligation efficiency of the S1-treated DNAs increased up to same level as the flush-ended DNA upon treatment with E. coli DNA polymerase I . Similar results were obtained in the case of S1 -generated unit-length linear duplex DNA. S1 does cleave both strands of superhelical DNA at unbasepaired sites.

References

Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·V SgaramellaH G Khorana

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Citations

Jul 17, 2008·Nucleic Acids Research·Omid R FaridaniLiam Good
Jun 5, 2007·BMC Biotechnology·Maxim PimkinAnthony T Yeung
Feb 15, 2003·FEMS Microbiology Reviews·Neelam A Desai, Vepatu Shankar
Jan 1, 1995·Critical Reviews in Microbiology·S U Gite, V Shankar

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