PMID: 9434001Jan 20, 1998Paper

Efficient adenoviral-mediated murine neonatal small intestinal gene transfer is dependent on alpha(v) integrin expression

Journal of Pediatric Surgery
T E HamiltonS E Raper

Abstract

Clinical application of gene therapy for patients who have inflammatory bowel disease or short bowel syndrome will require the development of new strategies to improve the efficiency of small intestinal gene transfer. Previously, the authors developed a method for adenoviral-mediated small intestinal gene transfer in vivo in neonatal and adult mice. The present study evaluates the hypothesis that the integrins alpha(v)beta3 and alpha(v)beta5, the secondary receptors for adenoviral internalization, play a facilitative role in neonatal murine adenoviral-mediated small intestinal gene transfer. Immunohistochemical techniques identified the integrin alpha(v)beta3 and the integrin subcomponents alpha(v), beta3, and beta5 in neonatal and adult small intestine. The effects of integrin receptor antagonists on transgene expression was also studied in our neonatal model of adenoviral-mediated small intestinal gene transfer in vivo. Gene transfer was significantly decreased by the addition of integrin receptor antagonists versus control peptide. Integrin alpha(v)beta3 and integrin subcomponent alpha(v), beta3, and beta5 are expressed in neonatal and adult small intestine. Integrin antagonists administered simultaneously blocked efficient ...Continue Reading

References

Jun 11, 1992·Nature·A D Miller
Feb 24, 1995·The Journal of Biological Chemistry·S KitazawaS L Teitelbaum
Sep 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·P Simon-AssmannM Kedinger
Jun 1, 1993·Current Opinion in Genetics & Development·K F Kozarsky, J M Wilson
Sep 1, 1995·Human Gene Therapy·C LauS J Henning

❮ Previous
Next ❯

Citations

Sep 14, 2001·Biochemical and Biophysical Research Communications·M MasumuraT Sawada
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·C A GuerreroC F Arias
May 14, 2016·World Journal of Virology·Carlos A Guerrero, Orlando Acosta
Nov 30, 2006·The Journal of Gene Medicine·Filippos KesisoglouEllen M Zimmermann

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.