Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy

Stem Cells International
Xinxin HanZhengliang Gao

Abstract

Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.

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Citations

Aug 15, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Cristina AlmenglóVíctor M Arce
Jun 9, 2020·BioMed Research International·Min WangXin-Xin Han
Feb 4, 2019·Current Neurology and Neuroscience Reports·Ania DabrowskiRyan J Felling
Dec 10, 2020·Stem Cells International·Kimia HosseiniRobert Fredriksson
Jul 25, 2021·International Journal of Molecular Sciences·Bing-Chun LiuSi-Qin Bao

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Methods Mentioned

BETA
flow cytometry
Assay

Software Mentioned

FlowJo
ImageJ
GraphPad Prism
Excel

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