Efficient and Low Cytotoxicity Gene Carriers Based on Amine-Functionalized Polyvinylpyrrolidone

Polymers
Anselmo Del PradoCarlos Elvira

Abstract

Non-viral vectors are a safety tool for gene therapy to deliver therapeutic genes. Among the different non-viral vectors, polyvinylpyrrolidone (PVP), a well-known hydrosoluble, neutral, and non-toxic polymer, satisfies the requirements and becomes a suitable candidate for gene delivery. In this study, we describe the preparation of polyvinylpyrrolidones decorated with pyrrolidine, piperidine, and piperazine groups, and evaluate them in vitro as non-viral gene carriers. The properties of these new systems are compared with those of hyperbranched polyethyleneimine (PEI) used as a positive control. Their ability to complex DNA at different N/P molar ratios, from 1:1 up to 10:1, was studied through agarose gel electrophoresis and dynamic light scattering. The resulting complexes (polyplexes) were characterized and evaluated in vitro with murine fibroblast (Swiss 3T3) as non-viral gene carriers, using luciferase as the reporter gene and a calcein cytocompatibility assay. All the copolymers condensed DNA to a particle average size between 100-400 nm when used at N/P ratios of 4:1 or higher. The copolymers with piperidine groups showed higher transfection efficiency than the pyrrolidine and piperazine modified copolymers, and even hig...Continue Reading

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Citations

Apr 4, 2021·Antibiotics·Marta O TeixeiraHelena P Felgueiras
Jun 5, 2021·International Journal of Biological Macromolecules·Divya SharmaBuddhadev Layek

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Methods Mentioned

BETA
transfection
Assay
fluorescence microscopy
electrophoresis
dynamic
electrophoretic light scattering
dynamic light scattering

Software Mentioned

- Correlator Control
Graph Path Prims
CellD
ALV

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