Efficient assembly of ribosomes is inhibited by deletion of bipA in Escherichia coli

Journal of Bacteriology
Promisree Choudhury, Ann M Flower

Abstract

The bacterial BipA protein belongs to the EF-G family of translational GTPases and has been postulated to be either a regulatory translation factor or a ribosome assembly factor. To distinguish between these hypotheses, we analyzed the effect of bipA deletion on three phenotypes associated with ribosome assembly factors: cold sensitivity, ribosome subunit distribution, and rRNA processing. We demonstrated that a ΔbipA strain exhibits a cold-sensitive phenotype that is similar to, and synergistic with, that of a strain with a known ribosome assembly factor, deaD. Additionally, the bipA deletion strain displayed a perturbed ribosome subunit distribution when grown at low temperature, similar to that of a deaD mutant, and again, the double mutant showed additive effects. The primary ribosomal deficiency noted was a decreased level of the 50S subunit and the appearance of a presumed pre-50S particle. Finally, deletion of bipA resulted in accumulation of pre23S rRNA, as did deletion of deaD. We further found that deletion of rluC, which encodes a pseudouridine synthase that modifies the 23S rRNA at three sites, suppressed all three phenotypes of the bipA mutant, supporting and extending previous findings. Together, these results sug...Continue Reading

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Citations

Aug 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Veerendra KumarYong-Gui Gao
May 6, 2016·Molecular Microbiology·Wieland Steinchen, Gert Bange
Mar 23, 2017·Molecular and Cellular Biology·Jalyce L E HellerHans-Joachim Wieden
Jul 15, 2015·The Journal of Biological Chemistry·Haitian FanGregor Blaha
Dec 2, 2020·Infection and Immunity·Samantha D CraneRoger D Pechous
Jul 28, 2020·Journal of Molecular Biology·Michelle R GibbsKurt Fredrick

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