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Efficient assessment of modified nucleoside stability under conditions of automated oligonucleotide synthesis: characterization of the oxidation and oxidative desulfurization of 2-thiouridine

Nucleosides, Nucleotides & Nucleic Acids

Nov 27, 2001

Elzbieta Sochacka

Abstract

In order to efficiently assess the chemical stability of modified nucleosides to the reagents and conditions of automated oligonucleotide synthesis, we designed, developed and tested a scheme in which the modified nucleoside, directly attached to a solid support, is exposed to the cycli...read more

Mentioned in this Paper

Carbon Tetrachloride
Dioxygen
Hydrogen Peroxide
RNA Chemical Synthesis
RNA Denaturation
Sulfur
Oxidation
High Pressure Liquid Chromatography Procedure
Oligonucleotides
Nucleosides
Paper Details
References
  • References6
  • Citations10
1

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Efficient assessment of modified nucleoside stability under conditions of automated oligonucleotide synthesis: characterization of the oxidation and oxidative desulfurization of 2-thiouridine

Nucleosides, Nucleotides & Nucleic Acids

Nov 27, 2001

Elzbieta Sochacka

PMID: 11720000

DOI: 10.1081/ncn-100107198

Abstract

In order to efficiently assess the chemical stability of modified nucleosides to the reagents and conditions of automated oligonucleotide synthesis, we designed, developed and tested a scheme in which the modified nucleoside, directly attached to a solid support, is exposed to the cycli...read more

Mentioned in this Paper

Carbon Tetrachloride
Dioxygen
Hydrogen Peroxide
RNA Chemical Synthesis
RNA Denaturation
Sulfur
Oxidation
High Pressure Liquid Chromatography Procedure
Oligonucleotides
Nucleosides

Trending Feeds

Blastomycosis

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Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Related Papers

Current Protocols in Nucleic Acid Chemistry

Preparation of short interfering RNA containing the modified nucleosides 2-thiouridine, pseudouridine, or dihydrouridine

Current Protocols in Nucleic Acid ChemistryJune 3, 2009
Barbara Nawrot, Elzbieta Sochacka
Nucleic Acids Research

Chemical incorporation of 1-methyladenosine into oligonucleotides

Nucleic Acids ResearchFebruary 28, 2002
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Paper Details
References
  • References6
  • Citations10
1
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