Efficient Bioconversion of Sucrose to High-Value-Added Glucaric Acid by In Vitro Metabolic Engineering

ChemSusChem
Hui-Hui SuWen-Yong Lou

Abstract

Glucaric acid (GA) is a major value-added chemicals feedstock and additive, especially in the food, cosmetics, and pharmaceutical industries. The increasing demand for GA is driving the search for a more efficient and less costly production pathway. In this study, a new in vitro multi-enzyme cascade system was developed, which converts sucrose efficiently to GA in a single vessel. The in vitro system, which does not require adenosine triphosphate (ATP) or nicotinamide adenine dinucleotide (NAD+ ) supplementation, contains seven enzymes. All enzymes were chosen from the BRENDA and NCBI databases and were expressed efficiently in Escherichia coli BL21(DE3). All seven enzymes were combined in an in vitro cascade system, and the reaction conditions were optimized. Under the optimized conditions, the in vitro seven-enzyme cascade system converted 50 mm sucrose to 34.8 mm GA with high efficiency (75 % of the theoretical yield). This system represents an alternative pathway for more efficient and less costly production of GA, which could be adapted for the synthesis of other value-added chemicals.

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Citations

Apr 9, 2020·International Journal of Molecular Sciences·Jorick Franceus, Tom Desmet
Mar 20, 2020·Biotechnology for Biofuels·Thu V Vuong, Emma R Master
Jan 8, 2021·Natural Product Reports·Yang LiXiulai Chen
Nov 15, 2019·Metabolic Engineering·Kerstin PetrollAnwar Sunna
Dec 12, 2020·Biotechnology for Biofuels·Lu-Zhou ChenJu-Zheng Sheng
Jul 9, 2021·Journal of Agricultural and Food Chemistry·Fu-Xing NiuJian-Zhong Liu
Aug 29, 2020·Journal of Agricultural and Food Chemistry·Chao LiHui-Min Qin
Jan 25, 2020·Journal of Agricultural and Food Chemistry·Fu-Xing NiuJian-Zhong Liu

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