Efficient cell penetration and delivery of peptide nucleic acids by an argininocalix[4]arene

Scientific Reports
Jessica GasparelloFrancesco Sansone

Abstract

The application of Peptide Nucleic Acids (PNAs), mimics of DNA lacking the sugar-phosphate backbone, for antisense/anti-gene therapy and gene editing is limited by their low uptake by cells. Currently, no simple and efficient delivery systems and methods are available to solve this open issue. One of the most promising approach is the modification of the PNA structure through the covalent linkage of poliarginine tails, but this means that every PNA intended to be internalized must be modified. Herein we report the results relative to the delivery ability of a macrocyclic multivalent tetraargininocalix[4]arene (1) used as non-covalent vector for anti-miR-221-3p PNAs. High delivery efficiency, low cytotoxicity, maintenance of the PNA biological activity and ease preparation of the transfection formulation, simply attained by mixing PNA and calixarene, candidate this vector as universal delivery system for this class of nucleic acid analogues.

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Citations

May 19, 2020·Expert Opinion on Drug Discovery·Meirav Segal, Frank J Slack
Sep 30, 2020·Molecular Biology Reports·Rohit PatelMeenu Saraf
Jul 28, 2020·Chemistry : a European Journal·Tania NevaJosé M García Fernández
Jan 28, 2021·Methods and Protocols·Ishani Dasgupta, Anushila Chatterjee
Dec 31, 2020·Pharmaceuticals·Stefano VolpiRoberto Corradini
Dec 19, 2020·Nanomaterials·Gonzalo Rivero-BarbarrojaJosé Manuel García Fernández
Apr 21, 2020·Current Pharmaceutical Biotechnology·Atieh Hashemi, Gilar Gorji-Bahri
Jul 14, 2021·Organic & Biomolecular Chemistry·Kaivin HadidiAlessandro Volonterio
Jul 17, 2021·Organic & Biomolecular Chemistry·Ilaria MorbioliFrancesco Sansone

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Methods Mentioned

BETA
transfection
PCR
transfect
FACS

Software Mentioned

CellQuest Pro

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