Efficient centrifugal recovery of Bacillus thuringiensis biopesticides from fermented wastewater and wastewater sludge

Water Research
Satinder K BrarR Y Surampalli

Abstract

Studies were conducted on harvesting of Bacillus thuringiensis (Bt)-based biopesticides from fermented broths of starch industry wastewater (SIW), wastewater sludge (raw and hydrolyzed-NH and TH, respectively) and semi-synthetic soyameal to enhance entomotoxicity (Tx) by centrifugation. Pertinent factors influencing Tx, solids concentration, pH, temperature and centrifugal force were investigated. The centrifugate solids concentration beyond 100 g/l did not enhance Tx, instead caused pellet formation. Centrifugation efficiency (Tx recovery) was higher at pH 4, and temperature 20 degrees C for starch wastewater (98%), wastewater sludge (98% and 97.8% for non-hydrolyzed and hydrolyzed, respectively) and soya broth (83%). For maximum Tx recovery (SIW-95%; NH-90%; TH-98% and soya-78%), the centrifugal force and time required was 48,000 g and 30 min, respectively. Losses in recovery efficiency were lower for SIW and wastewater sludge in comparison to soya on adopting commercially recommended centrifugal force of 9000 g. The settling velocity computations for different fermented broths enabled calculation of Sigma factor for continuous commercial centrifuge of a given capacity and hence simulation of power requirements. It was establ...Continue Reading

References

Nov 1, 1973·Biotechnology and Bioengineering·M W Tenney, F H Verhoff
Apr 1, 1993·The Journal of Nervous and Mental Disease·E S Gomberg
Sep 25, 2002·Journal of Bacteriology·Sinda FedhilaDidier Lereclus
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Jan 22, 2005·Journal of Industrial Microbiology & Biotechnology·A YezzaJ Smith
Jun 28, 2005·Water Research·S K BrarR Y Surampalli

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Citations

Jun 6, 2009·Environmental Technology·Khanh Dang VuR Y Surampalli
Sep 14, 2010·Bioprocess and Biosystems Engineering·K D AdjalléR Y Surampalli
Jan 24, 2016·Bioresource Technology·Gregory J O Martin
May 18, 2010·Journal of Basic Microbiology·María-Enriqueta Muñoz-Márquez, Elizabeth Ponce-Rivas
Sep 6, 2014·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Jean R GnepeRao Y Surampalli
Sep 25, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Adama NdaoJose Valéro

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