Efficient co-delivery of microRNA 21 inhibitor and doxorubicin to cancer cells using core-shell tecto dendrimers formed via supramolecular host-guest assembly.

Journal of Materials Chemistry. B, Materials for Biology and Medicine
Cong SongXiangyang Shi

Abstract

Development of versatile and powerful nanoplatforms for efficient therapeutic delivery represents a major topic for current nanomedicine. Herein, we present the development of core-shell tecto dendrimers (CSTDs) for co-delivery of a therapeutic gene and drug for enhanced anticancer therapy applications. In this work, CSTDs were first prepared via supramolecular recognition of β-cyclodextrin (CD)-decorated generation 5 (G5) poly(amidoamine) (PAMAM) dendrimers as cores and adamantane (Ad)-functionalized G3 PAMAM dendrimers as shell components. The formed CSTDs with each G5 dendrimer surrounded with 4.2 G3 dendrimers were evaluated as a gene vector for delivery of plasmid DNA encoding enhanced green fluorescent protein as well as microRNA 21 inhibitor (miR 21i). We show that under an appropriate N/P ratio, the CSTDs enable effective transfection of both genetic materials to cancer cells. In particular, the transfection of miR 21i led to the inhibition of cancer cell migration, decreased miR 21 gene expression, and the effective regulation of the target genes and proteins (e.g., PTEN, PDCD4, p53, and Caspase-3). Furthermore, we revealed that the CSTDs were able to co-deliver miR 21i and an anticancer drug doxorubicin, leading to en...Continue Reading

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Citations

Jul 18, 2020·Organic & Biomolecular Chemistry·Andrzej S SkwareckiMaria J Milewska
Sep 20, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Haonan LiKui Luo
Jan 19, 2021·Bioconjugate Chemistry·Shewaye Lakew MekuriaXiangyang Shi
Mar 7, 2021·International Journal of Molecular Sciences·Rebecca RaueBernhard Brüne
Apr 4, 2021·International Journal of Molecular Sciences·Piotr Tarach, Anna Janaszewska
Apr 4, 2021·Pharmaceutics·Veronica FollieroMassimiliano Galdiero
Dec 24, 2020·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Xihui GaoChangyou Zhan
May 12, 2021·Advanced Healthcare Materials·Deidra M WardNicholas A Peppas
May 25, 2021·Materials Today. Bio·Zhijun OuyangXiangyang Shi
Jun 5, 2021·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sunil Kumar DubeyPrashant Kesharwani
Jul 31, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Dayuan WangMingwu Shen
Jan 8, 2021·ACS Nano·Rebecca KaupAldrik H Velders

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Methods Mentioned

BETA
transfect
flow
confocal
transfection
atomic force microscopy
Assay
electrophoresis
AFM
PCR
flow cytometry

Software Mentioned

Image J

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