Efficient conditional transgene expression in hepatitis C virus cDNA transgenic mice mediated by the Cre/loxP system.

The Journal of Biological Chemistry
Takaji WakitaM Kohara

Abstract

Conditional gene expression has greatly facilitated the examination of the functions of particular gene products. Using the Cre/loxP system, we developed efficient conditional transgene activation of hepatitis C virus (HCV) cDNA (nucleotides 294-3435) in transgenic mice. Efficient recombination was observed in transgenic mouse liver upon intravenous administration of adenovirus that expresses Cre DNA recombinase. After transgene activation, most hepatocytes were stained with anti-core polyclonal antibody, and 21-, 37-, and 64-kDa proteins were detected by Western blot analysis in liver lysates using anti-core, E1, and E2 monoclonal antibodies, respectively. Serum core protein was detected in transgenic mice 7 days after transgene activation with concurrent increases in serum alanine aminotransferase levels. Subsequently, an anti-core antibody response was detected 14 days after infection. Furthermore, a CD4 and CD8 positive cell depletion assay normalized both the serum alanine aminotransferase increases and pathological changes in the liver. These results suggest that HCV proteins are not directly cytopathic and that the host immune response plays a pivotal role in HCV infection. Thus, this HCV cDNA transgenic mouse provides a...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P C OrbanJ D Marth
Oct 1, 1990·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·K KiyosawaR H Purcell
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N KatoK Shimotohno
Jun 10, 1988·Science·R Jaenisch
Sep 8, 1995·Science·R KühnK Rajewsky
Jun 1, 1994·Japanese Journal of Medical Science & Biology·Y KanegaeI Saito
Jun 1, 1994·Journal of Virology·E SantoliniN La Monica
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·Y K ShimizuH Yoshikura
Mar 1, 1996·Journal of Medical Virology·D MoradpourJ R Wands
Jan 7, 1997·Proceedings of the National Academy of Sciences of the United States of America·K KoikeT Miyamura
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·G BarbaC Bréchot
Mar 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C PasquinelliF V Chisari
Apr 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T KawamuraT J Liang
Jun 1, 1997·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M E Major, S M Feinstone

❮ Previous
Next ❯

Citations

Sep 29, 1999·Journal of Medical Virology·A HondaT Tokuhisa
Jul 19, 2002·Genesis : the Journal of Genetics and Development·Michael BadorfGudrun Schiedner
Sep 5, 2002·Reviews in Medical Virology·Susanne BeckebaumGuido Gerken
Mar 4, 2000·Journal of Dermatological Science·M Kohara
May 29, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A Y LeeX Paliard
Sep 5, 2002·The Journal of Experimental Medicine·Takafumi YoshidaAkihiko Yoshimura
Jun 15, 2001·ILAR Journal·M A Feitelson, J D Larkin
Feb 13, 2001·Nucleic Acids Research·I Dragatsis, S Zeitlin
Mar 30, 2011·Journal of Gastroenterology and Hepatology·Taro YamashitaShuichi Kaneko
Jul 15, 2005·Journal of Virology·Stefan F Wieland, Francis V Chisari
Nov 28, 2007·Annual Review of Pathology·Luca G Guidotti, Francis V Chisari
Oct 22, 2008·Annual Review of Pathology·David R McGivern, Stanley M Lemon
Nov 28, 2007·Uirusu·Saki KondoYumi Kanegae
Nov 16, 2011·ISRN Hematology·Kyoko Tsukiyama-KoharaMichinori Kohara
Jan 19, 2005·Journal of Viral Hepatitis·R Moreno-Otero
Jul 27, 2012·Cellular and Molecular Life Sciences : CMLS·Xiangyang Miao
Jun 24, 2008·Scandinavian Journal of Gastroenterology·Ming-Ling ChangDeng-Yn Lin
Apr 23, 2011·Experimental Animals·Kiminori Kimura, Michinori Kohara
Sep 25, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A SabileG Barba
Jan 27, 2009·Biochimica Et Biophysica Acta·John McLauchlan
Sep 8, 2007·Advanced Drug Delivery Reviews·Tsunamasa WatanabeMichinori Kohara
Sep 8, 2007·Journal of Hepatology·Tsunamasa WatanabeMichinori Kohara
Jul 14, 2007·Journal of Virological Methods·Batbayar TumurbaatarJiaren Sun
Nov 18, 2008·Chemical Biology & Drug Design·Reem BeleidKamaljit Kaur
Jul 24, 2013·Journal of Gastroenterology and Hepatology·Moses T BilityLishan Su
Jul 22, 2010·Journal of Medical Virology·Kenichi SatohMichinori Kohara
Feb 17, 2010·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Kazuhiko KoikeYoshiharu Matsuura
Dec 15, 2010·The Journal of General Virology·Juandy JoRobert Thimme
May 7, 2008·Journal of Hepatology·Heidi BarthThomas F Baumert

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

© 2022 Meta ULC. All rights reserved