PMID: 18714677Aug 22, 2008Paper

Efficient delivery of human single fiber-derived muscle precursor cells via biocompatible scaffold

Cell Transplantation
Luisa BoldrinPaolo De Coppi

Abstract

The success of cell therapy for skeletal muscle disorders depends upon two main factors: the cell source and the method of delivery. In this work we have explored the therapeutic potential of human muscle precursor cells (hMPCs), obtained from single human muscle fibers, implanted in vivo via micropatterned scaffolds. hMPCs were initially expanded and characterized in vitro by immunostaining and flow cytometric analysis. For in vivo studies, hMPCs were seeded onto micropatterned poly-lactic-glycolic acid 3D-scaffolds fabricated using soft-lithography and thermal membrane lamination. Seeded scaffolds were then implanted in predamaged tibialis anterior muscles of CD1 nude mice; hMPCs were also directly injected in contralateral limbs as controls. Similarly to what we previously described with mouse precursors cells, we found that hMPCs were able to participate in muscle regeneration and scaffold-implanted muscles contained a greater number of human nuclei, as revealed by immunostaining and Western blot analyses. These results indicate that hMPCs derived from single fibers could be a good and reliable cell source for the design of therapeutic protocols and that implantation of cellularized scaffolds is superior to direct injection...Continue Reading

References

Mar 1, 1977·Acta Pathologica Et Microbiologica Scandinavica. Section A, Pathology·F Melsen, L Mosekilde
Mar 1, 1991·Journal of Cellular Physiology·M R HathawayW R Dayton
Aug 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·H M BlauG K Pavlath
Nov 1, 1995·In Vitro Cellular & Developmental Biology. Animal·J D RosenblattT A Partridge
Apr 2, 1999·Science·M F PittengerD R Marshak
Dec 30, 1999·Biomaterials·R S KaneG M Whitesides
Feb 5, 2000·Developmental Biology·P Seale, M A Rudnicki
Jan 11, 2002·Blood Cells, Molecules & Diseases·B M DeasyJ Huard
Apr 4, 2002·In Vitro Cellular & Developmental Biology. Animal·Sylvie BonavaudGillian Butler-Browne
May 2, 2002·Journal of Nursing Management·Patrick A Crookes, Peter L Bradshaw
Apr 16, 2003·Biomaterials·Giovanni VozziSangeeta Bhatia
Sep 10, 2003·Experimental Hematology·Shannon L McKinney-FreemanMargaret A Goodell
May 29, 2004·American Journal of Physiology. Heart and Circulatory Physiology·M HorackovaZ Byczko
Jun 9, 2004·Journal of Pediatric Surgery·Julie R FuchsDario O Fauza
May 24, 2005·Cell and Tissue Banking·M Esther Rendal VázquezF J Blanco Garcia
Sep 6, 2005·Science·Didier MontarrasMargaret Buckingham
Sep 8, 2005·Tissue Engineering·Paolo De CoppiShay Soker
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Elliott HillDavid J Mooney
Jun 15, 2006·Tissue Engineering·Elliott HillDavid J Mooney
Jun 27, 2006·Nature Cell Biology·Vasily ShininShahragim Tajbakhsh
Sep 1, 2006·Experimental Gerontology·Sameer S JejurikarWilliam M Kuzon

❮ Previous
Next ❯

Citations

Aug 14, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jonathan M FishmanPaolo De Coppi
Jul 14, 2009·Tissue Engineering. Part B, Reviews·Hua Liao, Guang-Qian Zhou
Jan 30, 2014·Tissue Engineering. Part a·Kristin D McKeon-FischerJoseph W Freeman
Nov 30, 2010·Nature Materials·Richard T TranJian Yang
Oct 7, 2011·Expert Opinion on Biological Therapy·Jonathan M FishmanPaolo Macchiarini
Jun 23, 2011·Journal of Tissue Engineering and Regenerative Medicine·Marloes L P LangelaanFrank P T Baaijens
Nov 28, 2012·Advanced Healthcare Materials·Debanti SenguptaSarah C Heilshorn
Jul 7, 2010·Journal of Tissue Engineering and Regenerative Medicine·K J M BoonenM J Post
May 6, 2015·Critical Reviews in Food Science and Nutrition·Zuhaib Fayaz BhatHina Fayaz Bhat
Apr 25, 2012·Cell Transplantation·Iwona GrabowskaMaria A Ciemerych
Aug 17, 2018·International Journal of Molecular Sciences·Anna Urciuolo, Paolo De Coppi
Sep 30, 2013·Biomaterials Science·Anita F QuigleyRobert M I Kapsa
Jul 22, 2014·The International Journal of Artificial Organs·Chih-Hsun LinRuey-Yug Tsay
Jul 10, 2019·International Journal of Molecular Sciences·Iwona GrabowskaMaria A Ciemerych
Aug 23, 2020·Bioengineering·Mahdis Shayan, Ngan F Huang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.