Efficient delivery to human lung fibroblasts (WI-38) of pirfenidone incorporated into liposomes modified with truncated basic fibroblast growth factor and its inhibitory effect on collagen synthesis in idiopathic pulmonary fibrosis

Biological & Pharmaceutical Bulletin
Kohei TogamiSumio Chono

Abstract

In the present in vitro study, we assessed the delivery of pirfenidone incorporated into liposomes modified with truncated basic fibroblast growth factor (tbFGF) to lung fibroblasts and investigated the anti-fibrotic effect of the drug. The tbFGF peptide, KRTGQYKLC, was used to modify the surface of liposomes (tbFGF-liposomes). We used the thin-layer evaporation method, followed by sonication, to prepare tbFGF-liposomes containing pirfenidone. The cellular accumulation of tbFGF-liposomes was 1.7-fold greater than that of non-modified liposomes in WI-38 cells used as a model of lung fibroblasts. Confocal laser scanning microscopy showed that tbFGF-liposomes were widely localized in WI-38 cells. The inhibitory effects of pirfenidone incorporated into tbFGF-liposomes on transforming growth factor-β1 (TGF-β1)-induced collagen synthesis in WI-38 cells were evaluated by measuring the level of intracellular hydroxyproline, a major component of the protein collagen. Pirfenidone incorporated into tbFGF-liposomes at concentrations of 10, 30, and 100 µM significantly decreased the TGF-β1-induced hydroxyproline content in WI-38 cells. The anti-fibrotic effect of pirfenidone incorporated into tbFGF-liposomes was enhanced compared with that ...Continue Reading

References

Aug 1, 1965·Journal of Molecular Biology·A D BanghamJ C Watkins
Aug 1, 1997·The American Journal of Physiology·R BarriosM Selman
Aug 25, 2001·The New England Journal of Medicine·T J Gross, G W Hunninghake
Dec 28, 2004·Journal of Ethnopharmacology·Li-kun GongJin Ren
Oct 20, 2006·Journal of Drug Targeting·Takeshi TeradaMitsuru Hashida
May 1, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takeshi TeradaMitsuru Hashida
Oct 16, 2007·Chest·Chris J Scotton, Rachel C Chambers
Dec 10, 2009·The European Respiratory Journal·H TaniguchiUNKNOWN Pirfenidone Clinical Study Group in Japan
Jun 30, 2011·The Biochemical Journal·Jørgen WescheEllen Margrethe Haugsten
Oct 12, 2012·Drug Design, Development and Therapy·Huzaifa I Adamali, Toby M Maher
Jul 16, 2013·Current Opinion in Pulmonary Medicine·Amanda Belkin, Jeffrey J Swigris
Mar 13, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Enrico ConteCarlo Vancheri

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Citations

Jun 27, 2015·Journal of Liposome Research·Anup JoseVenkata Vamsi Krishna Venuganti
May 4, 2015·Biochemical and Biophysical Research Communications·Seo-Hyun ChoiYoon-Jin Lee
Mar 25, 2014·Pulmonary Pharmacology & Therapeutics·Hatem AlkhouriMichael Schuliga
Nov 14, 2018·Expert Opinion on Drug Delivery·Nasser AlhajjPaolo Colombo
Oct 30, 2018·Frontiers in Pharmacology·Leslie StradiotLeo A van Grunsven
Jun 23, 2019·Journal of Nanobiotechnology·Diego de la Fuente-HerreruelaIván López-Montero

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