Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples

Bioanalysis
Linda VårdalStig Pedersen-Bjergaard

Abstract

For the first time, extracts obtained from human plasma samples by electromembrane extraction (EME) were investigated comprehensively with particular respect to phospholipids using ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Thhe purpose was to investigate the potential of EME for phospholipid cleanup in different EME systems. No traces of phospholipids were detected in any of the acceptor solutions, whereas the model analytes were extracted with recoveries up to 50%. Thus, the EME systems tested in this work were found to be highly efficient for providing phospholipid-free extracts. Ultra-HPLC-MS/MS analysis of the donor solutions revealed that the phospholipids principally remained in the plasma samples. This proved that the phospholipids did not migrate in the electrical field and they were prevented from penetrating the supported liquid membrane.

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Citations

Jun 6, 2020·The Analyst·Magnus Saed RestanStig Pedersen-Bjergaard
Dec 8, 2017·The Analyst·Nicolas DrouinJulie Schappler
Jan 2, 2021·Forensic Science International : Synergy·Wing-Sum ChanBobbie Kwok-Keung Cheung
Feb 6, 2021·Bioanalysis·Linda Vårdal EieStig Pedersen-Bjergaard
Dec 11, 2020·Talanta·Hana Bavlovič PiskáčkováStig Pedersen-Bjergaard
Feb 4, 2021·Journal of Chromatography. a·Frederik André Hansen, Stig Pedersen-Bjergaard
Jul 7, 2018·Journal of Pharmaceutical and Biomedical Analysis·Nicolas DrouinJulie Schappler
May 30, 2019·Analytical Chemistry·Libin WanXiantao Shen

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Software Mentioned

EME
Xcalibur
Chromeleon

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