Efficient discrimination of Mycobacterium tuberculosis strains by 16S-23S spacer region-based random amplified polymorphic DNA analysis.

Journal of Clinical Microbiology
Y AbedP De Micco

Abstract

Amplification of the region separating the genes coding for 16S and 23S rRNA was performed with 15 Mycobacterium tuberculosis isolates and the type strain, ATCC 27294. Reproducible amplification patterns were obtained. PCR products were then used as target DNA for random amplified polymorphic DNA (RAPD) analysis. The discriminatory power was higher than when whole genomic DNA was used as a RAPD template. 16S-23S spacer region-based RAPD analysis was a simple and efficient method of differentiation. Consequently, it may be a useful tool for epidemiologic studies of tuberculosis.

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Citations

May 1, 1997·Memórias do Instituto Oswaldo Cruz·P N SuffysW M Degrave
Feb 15, 2014·BioMed Research International·Tomasz JagielskiJacek Bielecki
Oct 7, 2016·Journal of Korean Medical Science·Phyu Win EiChulhun L Chang
Apr 16, 2003·Journal of Applied Microbiology·E KandumaS H Gillespie
Oct 1, 1995·Journal of Clinical Microbiology·R Frothingham
Jan 1, 1997·Applied and Environmental Microbiology·M Vasquez, R T Espejo
Aug 31, 2002·Journal of Clinical Microbiology·Johanna MäkinenHanna Soini
May 26, 1999·The Journal of Infection·N A Saunders

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