Efficient dual sgRNA-directed large gene deletion in rabbit with CRISPR/Cas9 system

Cellular and Molecular Life Sciences : CMLS
Yuning SongLiangxue Lai

Abstract

The CRISPR RNA-guided Cas9 nuclease gene-targeting system has been extensively used to edit the genome of several organisms. However, most mutations reported to date have been are indels, resulting in multiple mutations and numerous alleles in targeted genes. In the present study, a large deletion of 105 kb in the TYR (tyrosinase) gene was generated in rabbit via a dual sgRNA-directed CRISPR/Cas9 system. The typical symptoms of albinism accompanied significantly decreased expression of TYR in the TYR knockout rabbits. Furthermore, the same genotype and albinism phenotype were found in the F1 generation, suggesting that large-fragment deletions can be efficiently transmitted to the germline and stably inherited in offspring. Taken together, our data demonstrate that mono and biallelic large deletions can be achieved using the dual sgRNA-directed CRISPR/Cas9 system. This system produces no mosaic mutations or off-target effects, making it an efficient tool for large-fragment deletions in rabbit and other organisms.

References

Aug 2, 2000·Mammalian Genome : Official Journal of the International Mammalian Genome Society·B AignerG Brem
May 6, 2003·Nature Biotechnology·David M ValenzuelaGeorge D Yancopoulos
Aug 4, 2010·Methods in Molecular Biology·Dmitry Y GuschinEdward J Rebar
Feb 18, 2012·Nature·Blake WiedenheftJennifer A Doudna
Jun 30, 2012·Science·Martin JinekEmmanuelle Charpentier
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Giedrius GasiunasVirginijus Siksnys
Mar 27, 2013·Cell Research·Nannan ChangJianzhong Jeff Xi
May 28, 2013·Genetics·Scott J GratzKate M O'Connor-Giles
Jul 3, 2013·Nature Methods·Ari E FriedlandJohn A Calarco
Jul 6, 2013·Cell Reports·Andrew R BassettJi-Long Liu
Jul 23, 2013·PloS One·Woong Y HwangJing-Ruey Joanna Yeh
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li-En JaoWenbiao Chen
Aug 10, 2013·Nature Biotechnology·Dali LiMingyao Liu
Nov 26, 2013·The International Journal of Biochemistry & Cell Biology·Jiankui ZhouXingxu Huang
Jan 31, 2014·Nature·Samuel H SternbergJennifer A Doudna
Feb 6, 2014·The FEBS Journal·Jiankui ZhouXingxu Huang
Apr 25, 2014·Nature Communications·Peter S Choi, Matthew Meyerson
Jun 1, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Seiya MizunoKen-ichi Yagami
Jul 2, 2014·Developmental Biology·Shuo-Ting YenRichard R Behringer
Aug 20, 2014·RNA Biology·Jinxiong HanWensheng Zhang
Nov 20, 2014·Cell Regeneration·Quanmei YanLiangxue Lai

❮ Previous
Next ❯

Citations

Feb 9, 2017·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Jayme Salsman, Graham Dellaire
Jun 7, 2018·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Jian FangQian Wang
Jun 9, 2016·Journal of Molecular Endocrinology·Suzy Markossian, Frédéric Flamant
Aug 21, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jichao DengZhanjun Li
Mar 4, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Tingjun LiuJian Q Feng
May 20, 2018·Archives of Pharmacal Research·Jong Geol LeeIn-Jeoung Baek
May 10, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhiquan LiuZhanjun Li
Jul 9, 2017·Biotechnology and Bioengineering·Catherine B MatthewsJ Christopher Love
Jan 13, 2017·Oncotarget·Yuning SongZhanjun Li
Oct 11, 2017·PloS One·Wenjun ZhouFeng Wang
Apr 29, 2019·Cellular and Molecular Life Sciences : CMLS·Zhiquan LiuLiangxue Lai
Oct 19, 2019·Scientific Reports·I Lamas-ToranzoP Bermejo-Álvarez
May 10, 2017·Scientific Reports·Yuning SongZhanjun Li
Jun 6, 2017·The Journal of Reproduction and Development·Arata Honda, Atsuo Ogura
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhiquan LiuZhanjun Li
Jan 22, 2020·Cell Death & Disease·Zhiquan LiuZhanjun Li
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·I Lamas-ToranzoP Bermejo-Álvarez
Jul 15, 2018·Nature Communications·Zhiquan LiuZhanjun Li
Jun 7, 2017·Plant Cell Reports·Ying WangXianggan Li
Oct 23, 2019·ELife·Ismael Lamas-ToranzoPablo Bermejo-Álvarez
Oct 3, 2020·Computational and Structural Biotechnology Journal·Yuanyuan Xu, Zhanjun Li
Oct 31, 2020·Journal of Molecular Cell Biology·Qiang Wu, Jia Shou
Jan 21, 2021·Animals : an Open Access Journal From MDPI·Xianbo JiaSongjia Lai
Feb 19, 2021·Laboratory Animals·Christophe Galichet, Robin Lovell-Badge
Feb 20, 2021·Frontiers in Genetics·Jianglin FanY Eugene Chen
Jul 3, 2021·Biology·Marlo K ThompsonAishwarya Prakash
Aug 31, 2021·Molecular Therapy. Nucleic Acids·Huanhuan ShanZhanjun Li

❮ Previous
Next ❯

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.