Efficient electrotransfection into canine muscle

Technology in Cancer Research & Treatment
Darja PavlinM Cemazar

Abstract

Two different types of electroporation protocols have been developed for efficient electrotransfer of plasmid DNA into skeletal muscle of experimental animals. At first, only low voltage electric pulses have been used, but lately, a combination of high and low voltage pulses has been suggested as more efficient. Up to date, in dogs, this type of electroporation protocol has never been used for muscle targeted plasmid DNA electrotransfection. In this study, we used two different DNA plasmids, one encoding green fluorescent protein and one encoding human interleukin-12. Five different electroporation protocols were evaluated. Three of them featured different combinations of high and low voltage pulses, and two were performed with delivery of low voltage pulses only. Our study shows that combination of 1 high voltage pulse (600 V/cm, 100 mus), followed by 4 low voltage pulses (80 V/cm, 100 ms, 1 Hz) yielded in the same transfection efficiency as the standard trains of low voltage pulses. However, this protocol is performed quicker and, thus, more suitable for potential use in clinical practice. In addition, it yielded in detectable systemic expression of human interleukin-12. Electrotransfer of either of the plasmids was associate...Continue Reading

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Citations

Mar 1, 2011·Radiology and Oncology·Darja PavlinNatasa Tozon
May 12, 2009·International Journal of Cancer. Journal International Du Cancer·Tien-Fu ChuangRea-Min Chu
Aug 21, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Ryan PersonRuxandra Draghia-Akli
May 11, 2010·Expert Review of Vaccines·Sylvia van Drunen Littel-van den Hurk, D Hannaman
Aug 13, 2011·American Journal of Physiology. Cell Physiology·Joseph A RocheRobert J Bloch
Nov 23, 2012·Journal of Translational Medicine·Darja PavlinNatasa Tozon

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