Efficient enrichment of hepatic cancer stem-like cells from a primary rat HCC model via a density gradient centrifugation-centered method.

PloS One
Wei-hui LiuKe-feng Dou

Abstract

Because few definitive markers are available for hepatic cancer stem cells (HCSCs), based on physical rather than immunochemical properties, we applied a novel method to enrich HCSCs. After hepatic tumor cells (HTCs) were first isolated from diethylinitrosamine-induced F344 rat HCC model using percoll discontinuous gradient centrifugation (PDGC) and purified via differential trypsinization and differential attachment (DTDA), they were separated into four fractions using percoll continuous gradient centrifugation (PCGC) and sequentially designated as fractions I-IV (FI-IV). Morphological characteristics, mRNA and protein levels of stem cell markers, proliferative abilities, induced differentiation, in vitro migratory capacities, in vitro chemo-resistant capacities, and in vivo malignant capacities were determined for the cells of each fraction. As the density of cells increased, 22.18%, 11.62%, 4.73% and 61.47% of primary cultured HTCs were segregated in FI-FIV, respectively. The cells from FIII (density between 1.041 and 1.062 g/ml) displayed a higher nuclear-cytoplasmic ratio and fewer organelles and expressed higher levels of stem cell markers (AFP, EpCAM and CD133) than cells from other fractions (P<0.01). Additionally, in v...Continue Reading

References

Oct 16, 2002·The Journal of Cell Biology·Atsushi AsakuraMichael A Rudnicki
Aug 5, 2003·Journal of Cellular Biochemistry·Melody BaddooDonald G Phinney
Jan 9, 2004·Proceedings of the National Academy of Sciences of the United States of America·Toru KondoTetsuya Taga
Oct 19, 2005·Cancer Research·Dong FangMeenhard Herlyn
Nov 16, 2005·Gastroenterology·Christopher M DekaneySusan J Henning
Jun 27, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tetsuhiro ChibaHideki Taniguchi
Oct 19, 2006·Experimental Cell Research·Annamaria HadnagyDanuta Balicki
Nov 14, 2006·Biochemical and Biophysical Research Communications·Atsushi SuetsuguHisataka Moriwaki
Nov 24, 2006·Nature·Lucia Ricci-VitianiRuggero De Maria
Dec 5, 2006·Stem Cell Reviews·Thomas Shupe, Bryon E Petersen
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·M E PrinceL E Ailles
Mar 9, 2007·Mutation Research·Igor P PogribnyOlga Kovalchuk
Jun 6, 2007·Proceedings of the National Academy of Sciences of the United States of America·Piero DalerbaMichael F Clarke
Jun 16, 2007·Gastroenterology·Stephanie MaXin-Yuan Guan
Jul 25, 2007·Annual Review of Cell and Developmental Biology·Neethan A LoboMichael F Clarke
Nov 2, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anna Cleta CroceGiovanni Bottiroli
Nov 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Masanori OnoYumi Matsuzaki
Dec 1, 2007·Cell Death and Differentiation·A EramoR De Maria
Jan 19, 2008·Nature·Tobias SchattonMarkus H Frank
Feb 5, 2008·Cancer Cell·Zhen Fan YangSheung Tat Fan
Feb 16, 2008·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Zhen Fan YangSheung Tat Fan
May 16, 2008·International Journal of Clinical Practice·W SongK Dou
May 24, 2008·The Journal of Clinical Investigation·Sergey V ShmelkovShahin Rafii
Jul 3, 2008·In Vitro Cellular & Developmental Biology. Animal·Gang ShenXiaofeng Yang
Jul 23, 2008·Molecular Cancer Research : MCR·Stephanie MaXin-Yuan Guan
Sep 9, 2008·Dermatologic Therapy·M R BongiornoM Aricò
May 6, 2009·Stem Cells·Shigeo TakaishiTimothy C Wang
Aug 29, 2009·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Tadashi Ikegami
Nov 17, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Wei-Hui LiuKe-Feng Dou
Mar 25, 2010·Biotechnology and Applied Biochemistry·Ana-Maria Rosca, Alexandrina Burlacu

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Citations

Apr 2, 2013·Stem Cells and Development·Jiang-Jie DuanShi-Cang Yu
Nov 28, 2012·Cellular Oncology (Dordrecht)·Wei-Hui LiuLi-Jun Tang
Nov 2, 2016·Preparative Biochemistry & Biotechnology·Narges RahmanianAmir-Hassan Zarnani
Feb 13, 2020·Signal Transduction and Targeted Therapy·Liqun YangHongjuan Cui
Aug 17, 2018·Canadian Journal of Gastroenterology & Hepatology·Leonardo do Prado LimaVivian Resende
Jan 13, 2021·Stem Cell Reviews and Reports·Lu Liu, Jürgen Borlak
Apr 17, 2020·Signal Transduction and Targeted Therapy·Liqun YangHongjuan Cui
May 6, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Danila CianciosiFrancesca Giampieri

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Methods Mentioned

BETA
density-gradient centrifugation
PCR
FACS
Light microscopy
xenograft

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