Efficient Fusion of Liposomes by Nucleobase Quadruple-Anchored DNA

Chemistry : a European Journal
Zhuojun MengAndreas Herrmann

Abstract

Anchoring DNA via hydrophobic units into the membrane of vesicles allows tagging of these nanocontainers with sequence information. Moreover, the hybridization of DNA on the surface of liposomes enables sequence specific functionalization, vesicle aggregation, and vesicle fusion. Specifically, DNA-hybridization-based approaches for fusion employing oligonucleotides terminally modified with one or two anchoring units were hindered by a limited degree of full fusion or by significant leakage during fusion. The current work deals with a new strategy for anchoring oligonucleotides on a membrane by lipid-modified nucleobases rather than by attaching hydrophobic units to the 3'- or 5'-termini. The lipid anchors were incorporated into the DNA sequence via phosphoramidite nucleotide building blocks during automated solid-phase synthesis allowing variation of the number and position of hydrophobic units along the DNA backbone. Single-stranded DNA functionalized with four lipid-modified nucleobases was stably grafted onto the membrane of lipid vesicles. It was found that the orientation of DNA hybridization and the number of anchoring units play a crucial role in liposomal fusion, which in the most efficient system reached remarkable 29 ...Continue Reading

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Citations

Jan 11, 2018·International Journal of Molecular Sciences·Niek S A CroneAimee L Boyle
May 14, 2019·Advanced Healthcare Materials·Hongyan LiAndreas Herrmann
Aug 8, 2018·International Journal of Molecular Sciences·Zhiyong ZhaoSimin Liu
May 28, 2019·Advanced Science·Shuaidong HuoAndreas Herrmann
Oct 19, 2017·Chemical Communications : Chem Comm·A RabeS Vogel
Oct 19, 2017·Organic & Biomolecular Chemistry·O RiesStefan Vogel
Oct 6, 2018·Chemical Science·Jian YangAlexander Kros
Jan 19, 2021·Advanced Healthcare Materials·Lingyu FengJianlei Shen
Mar 18, 2021·Advanced Biology·Nathaniel J Gaut, Katarzyna P Adamala
Mar 25, 2021·Macromolecular Bioscience·Jiafang PiaoYuanchen Dong
Mar 26, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Geert A DaudeyAlexander Kros
Oct 6, 2017·Langmuir : the ACS Journal of Surfaces and Colloids·Geert A DaudeyAimee L Boyle

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