PMID: 12763237May 24, 2003Paper

Efficient gene delivery to primary neuron cultures using a synthetic peptide vector system

Journal of Neuroscience Methods
Louise CollinsJohn W Fabre

Abstract

A bi-functional, 31 amino acid synthetic peptide (polylysine-molossin) was evaluated for gene delivery to primary cultures of rat cerebral cortex neurons. Polylysine-molossin consists of an amino terminal domain of 16 lysines for electrostatic binding of DNA, and a 15 amino acid, integrin-binding domain at the carboxyl terminal. High levels of gene delivery were obtained with 20-30 microM chloroquine, with a synthetic fusogenic peptide at an optimal DNA:polylysine-molossin:fusogenic peptide w/w ratio of 1:3:0.2, and with the addition of low concentrations of Lipofectamine 2000 at an optimal DNA:polylysine-molossin:Lipofectamine 2000 w/w ratio of 1:3:0.5. With the best combination, >30% of neurons strongly expressed the beta-galactosidase reporter gene, with no observable toxicity. DNA concentrations >2 microgram/ml were essential for efficient gene delivery. This synthetic peptide provides a safe, readily standardised and flexible DNA vector system well suited to ex vivo gene delivery to neurons for experimental and clinical applications.

References

Sep 15, 1990·Biochemical Pharmacology·P S TietzN F LaRusso
Jan 1, 1986·Annual Review of Biochemistry·I MellmanA Helenius
Apr 1, 1995·Neuron·D L PettitR Malinow
Feb 25, 1993·Nucleic Acids Research·P MidouxA C Roche
Jan 1, 1995·Ryōikibetsu shōkōgun shirīzu·M KaitoS Watanabe
Mar 1, 1997·Nature Medicine·D J Fink, J C Glorioso
Oct 1, 1995·Molecular Medicine Today·D A Treco, R F Selden
Jul 30, 1999·Nature Medicine·C Nyberg-Hoffman, E Aguilar-Cordova
May 29, 2000·Science·S R Wente
Apr 13, 2001·Neuron·K HaasH T Cline
Jun 16, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·C H MiaoX Ye
Jul 5, 2001·The Journal of Gene Medicine·S PatelJ W Fabre

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Citations

Nov 3, 2010·Nanomedicine·Inmaculada PosadasValentín Ceña
Jun 30, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Mohammadi KaouassDanuta Balicki
May 30, 2006·Biochimica Et Biophysica Acta·Martha L Arango-RodriguezDaniel Martinez-Fong
Feb 14, 2008·IEEE Transactions on Bio-medical Engineering·Tilak Jain, Jit Muthuswamy
Apr 22, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Elena SodduElisabetta Gavini
Dec 13, 2006·Journal of Peptide Science : an Official Publication of the European Peptide Society·Pan HaitaoGuo Xiaodong
Jun 22, 2007·The Journal of Gene Medicine·In-Kyu ParkSuzie H Pun
Jul 23, 2005·The Journal of Gene Medicine·Alan L ParkerJohn W Fabre

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