Efficient generation of insect-based cell-free translation extracts active in glycosylation and signal sequence processing

Journal of Biotechnology
Federico Katzen, Wieslaw Kudlicki

Abstract

A novel method for generation of insect-based cell-free translation extracts is presented. The protocol can be completed in less than an hour, and the resulting extracts are extremely proficient in N-linked glycosylation and signal sequence processing. No specialized equipment other than that usually present in an ordinary biochemistry laboratory is required. The novel approach dramatically reduces cost and time while rendering enhanced lysates compared to previously published strategies.

References

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Citations

Sep 12, 2007·Journal of Biomolecular NMR·Touraj Etezady-EsfarjaniKurt Wüthrich
Sep 4, 2008·Proteomics·Daniel SchwarzFrank Bernhard
Jun 27, 2013·Molecular Membrane Biology·Suzan Ruehrer, Hartmut Michel
Oct 16, 2016·Cold Spring Harbor Perspectives in Biology·Jessica G PerezMichael C Jewett
Jun 6, 2019·Methods and Protocols·Nicole E GregorioJavin P Oza
Oct 23, 2020·Journal of Industrial Microbiology & Biotechnology·Jasmine HersheweMichael C Jewett
Feb 20, 2021·Frontiers in Bioengineering and Biotechnology·Chiara HeideKaren M Polizzi
Apr 24, 2021·Nature Communications·Jasmine M HersheweMichael C Jewett
Aug 26, 2010·Journal of Proteome Research·Aarthi ChandrasekaranAnup K Singh

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