Efficient genetic transformation system for the ochratoxigenic fungus Aspergillus carbonarius.

Current Microbiology
Luiz Rodrigo I MoriokaMaria Helena P Fungaro

Abstract

Aspergillus carbonarius is a potent ochratoxin A producer that has been found in products such as grapes, coffee, spices, and cocoa. Ochratoxin A has nephrotoxic effect, and it has been classified as a possible carcinogenic substance for humans. Here we describe for the first time a transformation system for A. carbonarius, providing an important step towards its genetic manipulation. Conidia were transformed to acquire hygromycin B resistance using the AGL-1 strain of Agrobacterium tumefaciens. Genetic transformation was evaluated growing A. tumefaciens cells in induction medium containing or not acetosyringone prior to co-cultivation. The mean transforming efficiencies in IM+AS and IM-AS conditions were 62.2 and 44.5 transformants per 10(5) conidia, respectively. The hph gene was random integrated into the genome of A. carbonarius. Fungal sequences flanking the insertion site could be amplified by TAIL-PCR.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M E CaseN H Giles
Mar 13, 1965·Nature·K J van der MerweJ J Theron
Dec 1, 1965·Journal of the Chemical Society. Perkin Transactions 1·K J Van der MerweL Fourie
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
Jan 15, 1995·FEMS Microbiology Letters·M H FungaroJ L de Azevedo
Sep 22, 1998·Nature Biotechnology·M J de GrootA G Beijersbergen
Feb 21, 2002·Journal of Agricultural and Food Chemistry·Lucile SageEdmond E Creppy
Apr 19, 2002·Food Additives and Contaminants·Rocha Rosa C A DaA M Dalcero
Oct 10, 2002·International Journal of Food Microbiology·F J CabañesA Pons
Feb 6, 2003·International Journal of Food Microbiology·M H TaniwakiB T Iamanaka
Apr 17, 2003·Journal of Food Protection·Paola BattilaniZofia Kozakiewicz
Oct 2, 2003·Journal of Agricultural and Food Chemistry·Luigi BavarescoTerenzio Bertuzzi
Nov 25, 2003·Current Genetics·Andreas LeclerqueHans Ulrich Schairer
May 13, 2005·Current Genetics·Caroline B MichielseArthur F J Ram

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Citations

Jun 15, 2011·International Journal of Food Microbiology·A Crespo-SempereL González-Candelas
Oct 7, 2008·Journal of Zhejiang University. Science. B·Ji-Ye Wang, Hong-Ye Li
May 15, 2007·Canadian Journal of Microbiology·Marcia M MataMaria Helena P Fungaro
Aug 28, 2021·Applied Microbiology and Biotechnology·Xing ChenGong-Yuan Wei

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