Apr 8, 2020

PremPRI: Predicting the Effects of Single Mutations on Protein-RNA Interactions

BioRxiv : the Preprint Server for Biology
N. ZhangMinghui Li

Abstract

Protein-RNA interactions are crucial for many cellular processes, such as protein synthesis and regulation of gene expression. Missense mutations that alter protein-RNA interaction may contribute to the pathogenesis of many diseases. Here we introduce a new computational method PremPRI, which predicts the effects of single mutations occurring in RNA binding proteins on the protein-RNA interaction by calculating the binding affinity changes quantitatively. The multiple linear regression scoring function of PremPRI is composed of 11 sequence- and structure-based features, and is parameterized on 248 mutations from 50 protein-RNA complexes. Our model shows a good agreement between calculated and experimental values with Pearson correlation coefficient of 0.72 and the corresponding root-mean-square error of 0.76 kcal mol-1, and outperforms three other available methods. PremPRI can be used for finding functionally important variants, understanding the molecular mechanisms underlying the effects, and designing new protein-RNA interaction inhibitors. PremPRI is freely available at http://lilab.jysw.suda.edu.cn/research/PremPRI/.

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Mentioned in this Paper

Computer Software
Genome-Wide Association Study
Research
Genome
Papio
Babo protein, Drosophila
Nucleic Acid Sequencing
Aleurites fordii
Aleurites (plant)
Genetic Pedigree

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