Nov 8, 2018

Efficient GFP-labeling and analysis of spermatogenic cells using the IRG transgene and flow cytometry

BioRxiv : the Preprint Server for Biology
Leah L ZagoreDonny D Licatalosi

Abstract

Spermatogenesis is a highly ordered developmental program that produces haploid male germ cells. The study of male germ cell development in the mouse has provided unique perspectives into the molecular mechanisms that control cell development and differentiation in mammals, including tissue-specific gene regulatory programs. An intrinsic challenge in spermatogenesis research is the heterogeneity of germ and somatic cell types present in the testis. Techniques to separate and isolate distinct mouse spermatogenic cell types have great potential to shed light on molecular mechanisms controlling mammalian cell development, while also providing new insights into cellular events important for human reproductive health. Here, we detail a versatile strategy that combines Cre-lox technology to fluorescently label germ cells, with flow cytometry to discriminate and isolate germ cells in different stages of development for cellular and molecular analyses.

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Mentioned in this Paper

Study
Flow Cytometry
Benign Neoplasm of Testis
Research
Laboratory Procedures
Neoplasm of Uncertain or Unknown Behavior of Testis
IRG1
Cell Differentiation Process
Germ Cell Development
Haploid Cell

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