Efficient hepatic delivery and protein expression enabled by optimized mRNA and ionizable lipid nanoparticle

Bioactive Materials
Tongren YangYuanyu Huang

Abstract

mRNA is a novel class of therapeutic modality that holds great promise in vaccination, protein replacement therapy, cancer immunotherapy, immune cell engineering etc. However, optimization of mRNA molecules and efficient in vivo delivery are quite important but challenging for its broad application. Here we present an ionizable lipid nanoparticle (iLNP) based on iBL0713 lipid for in vitro and in vivo expression of desired proteins using codon-optimized mRNAs. mRNAs encoding luciferase or erythropoietin (EPO) were prepared by in vitro transcription and formulated with proposed iLNP, to form iLP171/mRNA formulations. It was revealed that both luciferase and EPO proteins were successfully expressed by human hepatocellular carcinoma cells and hepatocytes. The maximum amount of protein expression was found at 6 h post-administration. The expression efficiency of EPO with codon-optimized mRNA was significantly higher than that of unoptimized mRNA. Moreover, no toxicity or immunogenicity was observed for these mRNA formulations. Therefore, our study provides a useful and promising platform for mRNA therapeutic development.

Methods Mentioned

BETA
chemical modification
in vitro transcription
Confocal microscopy
Assay
dynamic light scattering
transmission electron microscopy
PCR
transfection
flow cytometry
ELISA

Clinical Trials Mentioned

NCT04283461

Software Mentioned

GraphPad
GraphPad Prism
FACS

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