Efficient human immunodeficiency virus (HIV-1) infection of cells lacking PDZD8

Virology
Shijian Zhang, Joseph Sodroski

Abstract

PDZD8 can bind the capsid proteins of different retroviruses, and transient knockdown of PDZD8 results in a decrease in the efficiency of an early, post-entry event in the retrovirus life cycle. Here we used the CRISPR-CAS9 system to create cell lines in which PDZD8 expression is stably eliminated. The PDZD8-knockout cell lines were infected by human immunodeficiency virus (HIV-1) and murine leukemia virus as efficiently as the parental PDZD8-expressing cells. These results indicate that PDZD8 is not absolutely necessary for HIV-1 infection and diminishes its attractiveness as a potential target for intervention.

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Citations

Jan 23, 2016·Pathogens·Claire D James, Sally Roberts
Sep 9, 2016·Nature Biotechnology·Rodolphe Barrangou, Jennifer A Doudna
Jun 7, 2019·Journal of Virology·Mohammad Adnan SiddiquiMasahiro Yamashita
Nov 7, 2019·Current Genetics·Antara BarmanSupriyo Chakraborty
Nov 4, 2017·Science·Yusuke HirabayashiFranck Polleux
Aug 26, 2018·Nature Reviews. Molecular Cell Biology·Carlotta GiorgiPaolo Pinton

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