Efficient immortalization of cells derived from critically endangered Tsushima leopard cat (Prionailurus bengalensis euptilurus) with expression of mutant CDK4, Cyclin D1, and telomerase reverse transcriptase

Cytotechnology
Ryo GoukoTomokazu Fukuda

Abstract

Tsushima leopard cat is the subspecies of Amur cats, and it is classified as the most highest class of critically endangered animals. Although the protection activity is highly recognized, the number of animals is decreasing due to the human activity and invasion of domestic cats and infectious disease. In this study, we succeeded primary culture of normal fibroblasts derived from the Tsushima leopard cat (Prionailurus bengalensis euptilurus). Furthermore, we introduced the human derived mutant Cyclin Dependent Kinase 4, Cyclin D1, and telomere reverse transcriptase. We showed that the expression of these three genes efficiently immortalized cells derived from Tsushima leopard cat. Furthermore, we showed that the chromosome pattern of the established cells is identical with the original one. These data indicate that our method of immortalization is useful to establish cell lines from critically endangered cats, which potentially contributes to the re-generation of critically endangered animals from cultured cell with reproductive technique, such as somatic cloning.

References

Mar 1, 1987·The Journal of Heredity·D H Wurster-HillY Ono
Dec 1, 1961·Experimental Cell Research·L HAYFLICK, P S MOORHEAD
Nov 15, 2012·In Vitro Cellular & Developmental Biology. Animal·Tomokazu FukudaMiho Inoue-Murayama
Aug 27, 2013·Analytical Biochemistry·Kenichiro DonaiTomokazu Fukuda
Dec 7, 2013·In Vitro Cellular & Developmental Biology. Animal·Tomokazu FukudaMiho Inoue-Murayama

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