Efficient in vitro and in vivo gene regulation of a retrovirally delivered pro-apoptotic factor under the control of the Drosophila HSP70 promoter

Gene Therapy
G RomanoR Baserga

Abstract

We have developed a self-inactivating retroviral vector system with an internal, inducible Drosophila HSP70 promoter. This vector system delivers the desired transgene into cells rapidly and efficiently. It generates mixed populations of transduced cells where the transgene is inducible, and does not require the isolation of specific clones. Since the transgene is not expressed (or poorly expressed) at the restrictive condition (34 degrees C), mixed populations can be selected in which tumor suppressors or other inhibitory genes can be strongly induced upon changing the conditions (39 degrees C or the plant amino acid L-canavanine). This retroviral vector should be very useful for the expression of sequences that are poorly tolerated by cells, and is also active in animals.

References

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Citations

Jun 28, 2003·American Journal of Respiratory Cell and Molecular Biology·Jiming ZhouRama K Mallampalli
Mar 1, 2005·Oncogene·Min ZhangArnab Chakravarti
Jun 27, 2007·Journal of Neuroscience Research·Jin Ying WangKrzysztof Reiss
Nov 17, 2005·Oncogene·Davide Ruggero, Nahum Sonenberg
Sep 15, 2005·Oncogene·Nicolle M LinnerthRoger A Moorehead
Mar 13, 2014·Acta Histochemica Et Cytochemica·Taketo SusaToshiyuki Matsuzaki

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