Efficient in vitro and in vivo pulmonary delivery of nucleic acid by carbon dot-based nanocarriers

Biomaterials
Philippe PierratLuc Lebeau

Abstract

Cationic carbon dots were fabricated by pyrolysis of citric acid and bPEI25k under microwave radiation. Various nanoparticles were produced in a 20-30% yield through straightforward modifications of the reaction parameters (stoichiometry of the reactants and energy supply regime). Particular attention was paid to the purification of the reaction products to ensure satisfactory elimination of the residual starting polyamine. Intrinsic properties of the particles (size, surface charge, photoluminescence and quantum yield) were measured and their ability to form stable complexes with nucleic acid was determined. Their potential to deliver plasmid DNA or small interfering RNA to various cell lines was investigated and compared to that of bPEI25k. The pDNA in vitro transfection efficiency of these carbon dots was similar to that of the parent PEI, as was their cytotoxicity. The higher cytotoxicity of bPEI25k/siRNA complexes when compared to that of the CD/siRNA complexes however had marked consequences on the gene silencing efficiency of the two carriers. These results are not fully consistent with those in some earlier reports on similar nanoparticles, revealing that toxicity of the carbon dots strongly depends on their protocol of...Continue Reading

References

Feb 1, 1994·Biophysical Journal·W M SaltzmanR A Cone
Dec 24, 1997·Bioconjugate Chemistry·M A ZantaJ P Behr
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·C L DensmoreV Knight
Sep 22, 2001·Biophysical Journal·S S OlmstedR A Cone
Jan 4, 2003·Blood·Christopher BaumChristof von Kalle
Jan 17, 2003·The New England Journal of Medicine·Salima Hacein-Bey-AbinaAlain Fischer
Jan 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Samuel K LaiJustin Hanes
Jul 21, 2007·International Journal of Pharmaceutics·Haruna SakuraiHiroyuki Mizuguchi
Feb 29, 2008·Journal of Pharmaceutical Sciences·Uta Wattendorf, Hans P Merkle
May 27, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Lee A DaviesDeborah R Gill
Oct 29, 2008·Chemical Communications : Chem Comm·Qiao-Ling ZhaoDai-Wen Pang
Dec 5, 2008·Chemical Reviews·Meredith A Mintzer, Eric E Simanek
Oct 15, 2009·International Journal of Pharmaceutics·Johannes GeigerCarsten Rudolph
Mar 17, 2010·Journal of the American Chemical Society·Oleksandr A KucherakAndrey S Klymchenko
May 8, 2010·Chemical Communications : Chem Comm·Hui ZhuXiurong Yang
Aug 6, 2010·Angewandte Chemie·Sheila N Baker, Gary A Baker
Nov 15, 2011·Chemical Communications : Chem Comm·Amit JaiswalArun Chattopadhyay
Dec 24, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Moritz Beck-BroichsitterThomas Kissel
Jul 6, 2012·Chemical Communications : Chem Comm·Xinyun ZhaiWenguang Liu
Feb 2, 2013·Pharmaceutical Research·Philippe PierratLuc Lebeau
Feb 7, 2013·Biomaterials·Benjamin S SchusterJustin Hanes
Aug 6, 2013·Accounts of Chemical Research·Changqin DingYang Tian
May 6, 2014·The European Respiratory Journal·Sabine H van RijtSilke Meiners

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Citations

Jun 5, 2016·Talanta·Li-Ming Shen, Jing Liu
Oct 23, 2016·Talanta·Quan-Xing MaoJian-Hua Wang
Oct 30, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Fatemeh Ostadhossein, Dipanjan Pan
Oct 28, 2016·ACS Applied Materials & Interfaces·Jingjing YangFu-Gen Wu
Dec 23, 2015·Scientific Reports·Yun Kyung JungByeong-Su Kim
Oct 20, 2018·AAPS PharmSciTech·Jing ChenYushun Dou
Feb 6, 2019·Nanomaterials·Cindy DiasBegoña Espiña
Jan 11, 2017·Journal of Nanobiotechnology·Fabian BlankBarbara Rothen-Rutishauser
Apr 3, 2020·Current Pharmaceutical Design·Eemaan N CohenViness Pillay
May 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiang ZhengXing-Jie Liang
Oct 10, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Tao WangRakesh N Veedu
Oct 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xi WenYa-Ping Guo
Mar 13, 2018·Beilstein Journal of Nanotechnology·Nagamalai VasimalaiMaría Teresa Fernández-Argüelles
Jan 3, 2021·Advances in Colloid and Interface Science·Mahnaz AmiriMasoud Salavati-Niasari
Jan 1, 2021·Annales pharmaceutiques françaises·S DugamN Jadhav
Apr 4, 2021·Nanomaterials·Helena B A SousaJoão A V Prior
Oct 18, 2020·Nanomedicine : Nanotechnology, Biology, and Medicine·Se-Jin ParkDal-Hee Min
Dec 22, 2016·ACS Applied Materials & Interfaces·Sristi MajumdarDevasish Chowdhury
Nov 18, 2017·ACS Applied Materials & Interfaces·Yun-Xia SunXian-Zheng Zhang
Jun 25, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Hua QiaoJun Xie
Sep 28, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ping ChenXiao-Qi Yu

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