Efficient In Vitro Electropermeabilization of Reconstructed Human Dermal Tissue

The Journal of Membrane Biology
Moinecha MadiLaure Gibot

Abstract

DNA electrotransfer is a successful technic for gene delivery. However, its use in clinical applications is limited since little is known about the mechanisms governing DNA electrotransfer in the complex environment occurring in a tissue. The objectives of this work were to investigate the role of the extracellular matrix (ECM) in that process. Tumor ECM composition was shown to modulate in vivo gene electrotransfer efficiency. In order to assess the effects of ECM composition and organization, as well as intercellular junctions and communication, in normal tissue response to electric pulses, we developed an innovative three-dimensional (3D) reconstructed human connective tissue model. 3D human dermal tissue was reconstructed in vitro by a tissue engineering approach and was representative of in vivo cell organization since cell-cell contacts were present as well as complex ECM. This human cell model presented multiple layers of primary dermal fibroblasts embedded in a native, collagen-rich ECM. This dermal tissue could become a useful tool to study skin DNA electrotransfer mechanisms. As proof of the concept, we show here that the cells within this standardized 3D tissue can be efficiently electropermeabilized by milliseconds ...Continue Reading

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Citations

Mar 21, 2020·Expert Opinion on Drug Delivery·Janja Dermol-ČerneDamijan Miklavčič
Jun 8, 2017·PloS One·Haiyan ChenYaojiong Wu
Aug 23, 2021·Biomedical Engineering Online·Saša Haberl Meglič, Mojca Pavlin

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