Efficient in vitro generation of adult multipotent cells from mobilized peripheral blood CD133+ cells.

Cell Proliferation
Selim KuciR Handgretinger

Abstract

To generate non-haematopoietic tissues from mobilized haematopoietic CD133(+) stem cells. Mobilized peripheral blood CD133(+) cells from adult healthy donors were used. In vitro ability of highly enriched CD133(+) cells from mobilized peripheral blood to generate multipotent cells, and their potential to give rise to cells with characteristics of neuroectoderm, endoderm and mesoderm layers was investigated. We found that a recently identified population of CD45(+) adherent cells generated in vitro after culture of highly purified CD133(+) cells for 3-5 weeks with Flt3/Flk2 ligand and interleukin-6 can, in presence of the appropriate microenvironmental cues, differentiate into neural progenitor-like cells (NPLCs), hepatocyte-like cells and skeletal muscle-like cells. We have termed them to be adult multipotent haematopoietic cells (AMHCs). AMHC-derived NPLCs expressed morphological, phenotypic and molecular markers associated with primary neural progenitor cells. They can differentiate into astrocyte-like cells, neuronal-like cells and oligodendrocyte-like cells. Moreover, AMHC-derived NPLCs produced 3,4-dihydrophenylalanine and dopamine and expressed voltage-activated ion channels, suggesting their functional maturation. In add...Continue Reading

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Citations

Jan 24, 2013·Stem Cells Translational Medicine·Maria Teresa González-GarzaJorge E Moreno-Cuevas
Dec 4, 2010·Neurochemical Research·George H De Vries, Anne I Boullerne
Mar 24, 2009·Birth Defects Research. Part C, Embryo Today : Reviews·Antonin BukovskyFei Xu
Jul 28, 2017·American Journal of Alzheimer's Disease and Other Dementias·Nehru Sai Suresh ChalichemBasavan Duraiswamy
Jan 31, 2018·Journal of Drug Targeting·Nehru Sai Suresh ChalichemDuraiswamy Basavan
Jun 3, 2021·Cells·Ranjithkumar RajendranMartin Berghoff

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