PMID: 15250046Jul 14, 2004Paper

Efficient induction of dopaminergic neurons from embryonic stem cells for application to Parkinson's disease

Yonsei Medical Journal
Dong-Wook Kim

Abstract

For cell replacement therapy of neurodegenerative diseases such as Parkinson's disease (PD), methods for efficiently generating midbrain dopaminergic (DA) neurons from embryonic stem (ES) cells have been investigated. Two aspects of DA neuron generation are considered: genetic modification and manipulation of culture conditions. A transcription factor known as critical for development of DA neurons, Nurr1, was introduced into ES cells to see how they facilitate the generation of DA neurons from ES cells. Also, two culture procedures, the 5-stage method and stromal cell-derived inducing activity (SDIA) method, were used for ES cell differentiation. Using the 5-stage method, we and others previously demonstrated that Nurr1-overexpressing ES cells, under treatment of signaling molecules such as SHH and FGF8 followed by treatment of ascorbic acid, can differentiate into DA neurons with a high efficiency (> 60% of TH+/Tuj1+ neurons). Furthermore, using the SDIA method with treatment of signaling molecules, we found that Nurr1-overexpressing ES cells can differentiate to DA neurons with the highest efficiency ever reported (approximately 90% of TH+/Tuj1+ neurons). Importantly, our semi-quantitative and real-time PCR analyses demonstr...Continue Reading

Citations

Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Yi WangWei-dong Le
Mar 26, 2005·Brain Research. Molecular Brain Research·Kai-Christian SonntagOle Isacson
Nov 7, 2012·Cell Transplantation·Hueng-Chuen FanShinn-Zong Lin
Mar 10, 2010·Regenerative Medicine·Rosemary A Fricker-Gates, Monte A Gates
Mar 28, 2006·Molecular & Cellular Proteomics : MCP·Jinghua JinJing Zhang
Jun 13, 2006·Biochemical and Biophysical Research Communications·Anand S SrivastavaEwa Carrier

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