Efficient induction of extrinsic cell death by dandelion root extract in human chronic myelomonocytic leukemia (CMML) cells.

PloS One
P OvadjeSiyaram Pandey

Abstract

Chronic Myelomonocytic Leukemia (CMML) is a heterogeneous disease that is not only hard to diagnose and classify, but is also highly resistant to treatment. Available forms of therapy for this disease have not shown significant effects and patients rapidly develop resistance early on in therapy. These factors lead to the very poor prognosis observed with CMML patients, with median survival duration between 12 and 24 months after diagnosis. This study is therefore centered around evaluating the selective efficacy of a natural extract from dandelion roots, in inducing programmed cell death in aggressive and resistant CMML cell lines. To confirm the induction of programmed cell death in three human CMML cell lines, nuclear condensation and externalization of the phosphatidylserine, two main characteristics of apoptosis, were detected using Hoechst staining and annexin-V binding assay. The induction of another mode of cell death, autophagy, was determined using a monodansylcadaverine (MDC) stain, to detect the formation of autophagy vacuoles. The results from this study indicate that Dandelion Root Extract (DRE) is able to efficiently and selectively induce apoptosis and autophagy in these cell lines in a dose and time dependent ma...Continue Reading

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Citations

Jul 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Rosa Moo-PucEdgar Caamal-Fuentes
Apr 18, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Pamela OvadjeSiyaram Pandey
May 29, 2018·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Tianhao BaoChunjie Xiao
Aug 9, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Christopher NguyenSiyaram Pandey

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Methods Mentioned

BETA
Fluorescence
protein assay

Software Mentioned

Adobe Photoshop
GraphPad Prism

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